Enzyme Gene Mutations Sequences and Patent Acquisition Strategies

Q: A ketoreductase enzyme has undergone a gene mutation at a certain mutation site and has acquired a mutant enzyme with higher activity. How can one search for publicly available sequences of such enzyme mutants and other popular mutation sites?

A: The sequence variations can be viewed through the Variation Report in the Patsnap Bio Sequence Database.

First, Create a free account in the Patsnap Bio Sequence Database. Enter the original enzyme's sequence in the regular search box on the database homepage and set the mutation site in the variation filter at the lower right corner.

After setting the parameters, click search to find related sequences, patents, literature, and data from other sources for the enzyme mutant. Moreover, you can further narrow down the search by setting sequence similarity and target sequence coverage range in the sequence filter options on the left side of the page to refine the search results.

Further clicking on the Variation Report at the top right of the sequence results gives you an overview report of the variations, and through the heatmap analysis module in the report, you can clearly see the other popular mutation sites in the original sequence. The darker the annotation color, the greater the extent of variation.

At the bottom of the Variation Report, each mutation site's type of variation and a description of the mutation are displayed. Additionally, an Export Report button in the upper right corner of the report allows you to export the report in a Word version for offline editing.

It is important to note that Patsnap Bio is the most extensive sequence search platform for the Patsnap database. It incorporates AI with human-curated data for comprehensive handling of protein and nucleotide sequence data plucked from global patents, biological periodicals, and public repositories. Essential biological sequences are manually annotated, illuminating structural modifications to provide the most accurate sequence data and boost sequence retrieval efficiency.

Free registration is available for the Bio biological sequence database: https://bio-patsnap-com.libproxy1.nus.edu.sg. Act now to expedite your sequence search tasks.