Circulating Neoplastic Cells

PARSORTIX SYSTEM AND ASSAYS BEING SHOWCASED AT A LEADING EUROPEAN BREAST CANCER CONFERENCE ANGLE presenting two posters highlighting the utility of the Company's new Portrait+ CTC Staining Kit and Portrait HER2 assay GUILDFORD, SURREY / ACCESSWIRE / March 22, 2024 / ANGLE plc (AIM:AG)(OTCQX:ANPCY), a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) solutions for use in research, drug development and clinical oncology, is pleased to announce its participation at the 14th European Breast Cancer Conference (EBCC) being held in Milan, Italy on 20 to 22 March 2024. EBCC is focused on multidisciplinary, high-quality clinical and translational research, with an emphasis on innovation and technology in the management of breast cancer. The conference is attended by researchers, physicians, patient advocates and industry representatives providing sales, partnership and collaboration opportunities. ANGLE is presenting two posters at the conference: 1. Integrating isolation using label-independent microfluidics and advanced staining for comprehensive Circulating Tumour Cell Analysis ANGLE's Parsortix® system and Portrait®+ CTC Staining Kit together provide an optimised, efficient, and standardised solution for the harvest and characterisation, via immunofluorescence (IF) staining, of a diverse range of CTC phenotypes. In the presented data, half of the CTC-positive breast cancer patient blood samples contained only mesenchymal CTCs, while half had a combination of both epithelial and mesenchymal CTCs. Patients with purely mesenchymal CTCs would have been missed by epitope-dependent systems. This is important because mesenchymal CTCs or CTCs undergoing epithelial-to-mesenchymal transition are clinically significant, playing a critical role in cancer progression, treatment resistance and metastasis. CTC clusters harvested by the Parsortix system were observed in 75% of the CTC-positive patients which is crucial given that CTC clusters have been shown to have up to 100 times the increased metastatic potential compared to single CTCs. The Parsortix cassette's unique design is suited to capturing and harvesting clusters which is often difficult with other CTC systems. The study results highlight the importance of using ANGLE's Parsortix system combined with the Company's Portrait+ CTC Staining Kit, to enable the isolation and analysis of a range of CTC phenotypes and CTC clusters, both of which are important in cancer progression and metastasis. 2. Interrogating HER2 status in Circulating Tumour Cells isolated using the Parsortix® system from Metastatic Breast Cancer patients The diagnosis and treatment of breast cancer is currently guided by the evaluation of human epidermal growth factor receptor 2 (HER2) status in tissue biopsies. However, a tissue biopsy is invasive, is at a single point in time and, with little opportunity for repeat testing, can become out of date. The longitudinal monitoring of HER2 expression from blood is possible through minimally invasive CTC-based liquid biopsy techniques. ANGLE has developed an IF assay for HER2 protein identification on CTCs isolated and harvested using the Parsortix system from the peripheral blood of metastatic breast cancer (MBC) patients. Results from an in-house study in 26 patients found that CTCs overexpressing HER2 were present at similar levels in patients that had HER2+ or HER2- tumours by primary tissue biopsy. HER2 status of CTCs was also dynamic, changing over time in 37% of CTC positive donors which is unsurprising given that temporal changes in HER2 tissue status are also well documented in the literature. One third-party study of 575 breast cancer patients similarly found that 38% had changed HER2 status from the original primary tissue biopsy to the metastatic tissue biopsy, at disease recurrence. These initial results using Parsortix-based harvest of CTCs demonstrate how the HER2 status between primary tumours and CTCs can diverge, and that analysis of HER2 expression on CTCs has the potential to help guide clinical decision making in MBC patients, allowing for regular monitoring and tracking of treatment and/or disease progression. ANGLE Chief Scientific Officer, Karen Miller, commented: "We are delighted to have had two posters presented at the EBCC conference. The results of these studies further demonstrate the potential utility of ANGLE's sample-to-answer Parsortix system and Portrait imaging assays for reliable and repeatable assessment of biomarkers in a range of solid tumours, including breast. We look forward to discussions with translational researchers and industry representatives at this conference to explore opportunities for sales, collaboration, and partnerships." ANGLE plc +44 (0) 1483 343434 Andrew Newland, Chief Executive Ian Griffiths, Finance Director Berenberg (NOMAD and Broker) Toby Flaux, Ciaran Walsh, Milo Bonser +44 (0) 20 3207 7800 FTI Consulting Simon Conway, Ciara Martin Matthew Ventimiglia (US) +44 (0) 203 727 1000 +1 (212) 850 5624 For Research Use Only. Not for use in diagnostic procedures. For Frequently Used Terms, please see the Company's website on Notes for editors About ANGLE plc ANGLE is a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) solutions for use in research, drug development and clinical oncology using a simple blood sample. ANGLE's FDA cleared and patent protected circulating tumour cell (CTC) harvesting technology known as the Parsortix® PC1 System enables complete downstream analysis of the sample including whole cell imaging and proteomic analysis and full genomic and transcriptomic molecular analysis. ANGLE's commercial businesses are focusing on diagnostic products and clinical services. Diagnostic products include the Parsortix® system and associated consumables. The clinical services business is offered through ANGLE's GCP-compliant laboratories in the UK. Services include custom made assay development and clinical trial testing for pharma. Over 90 peer-reviewed publications have demonstrated the performance of the Parsortix system. For more information, visit This information is provided by Reach, the non-regulatory press release distribution service of RNS, part of the London Stock Exchange. Terms and conditions relating to the use and distribution of this information may apply. For further information, please contact rns@lseg.com or visit . SOURCE: ANGLE plc View the original press release on accesswire.com

USE OF PARSORTIX SYSTEM IN PRE-CLINICAL MODEL UNLOCKS OPPORTUNITIES FOR DRUG DISCOVERY Harvested CTCs reveal gene expression pro with development and progression of brain metastasis in melanoma Researchers suggest such profiling could prove to be "pivotal" to prescribing more targeted treatments in this setting GUILDFORD, SURREY / ACCESSWIRE / December 11, 2023 / ANGLE plc (AIM:AGL)(OTCQX:ANPCY), a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) diagnostic solutions for use in research, drug development and clinical oncology, is delighted to announce the publication of breakthrough work by researchers at the University of New Mexico, US, into the role of CTC gene expression in the progression of melanoma brain metastasis. The researchers used the Parsortix system to harvest CTCs for analysis and concluded that "… gene expression in CTCs could be pivotal to prescribing more targeted treatment based on the needs of the patient." In this study, pre-clinical mouse models of melanoma brain metastasis were split into four treatment groups and two cancer drugs were tested individually and together vs. placebo. Blood samples were analysed using the Parsortix system to harvest CTCs. The authors found that using the two drugs together was the most effective therapeutic regime, reducing tumour burden, metastasis and CTC count. This research provides further evidence of the capability of the Parsortix system to be used for the harvesting of CTCs for analysis in pre-clinical mouse models. The global market for preclinical clinical research organisations exceeded a valuation of US$ 5 billion in 2022 and is expected to achieve a 7.5% CAGR over the next 10 years owing to increasing R&D expenditure on early-stage development combined with the growing number of drugs in preclinical development. ANGLE Chief Executive Officer, Andrew Newland, commented: "The use of CTC biomarkers in clinical trials is a rapidly growing field enabling longitudinal monitoring of transcriptomic and proteomic changes. ANGLE can provide pharma customers with a complete range of services to support preclinical and clinical drug development. This includes standardised and validated protocols for analysing CTCs including the established techniques of IF, dPCR and NGS, and the capability to deliver bespoke assays to identify drug targets and ascertain therapeutic efficacy." This peer-reviewed journal article is published in the journal of Cancers and available at . ANGLE plc +44 (0) 1483 343434 Andrew Newland, Chief Executive Ian Griffiths, Finance Director Berenberg (NOMAD and Broker) Toby Flaux, Ciaran Walsh, Milo Bonser +44 (0) 20 3207 7800 FTI Consulting Simon Conway, Ciara Martin Matthew Ventimiglia (US) +44 (0) 203 727 1000 +1 (212) 850 5624 For Frequently Used Terms, please see the Company's website on Notes for editors About ANGLE plc ANGLE is a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) diagnostic solutions for use in research, drug development and clinical oncology using a simple blood sample. ANGLE's FDA cleared and patent protected circulating tumour cell (CTC) harvesting technology known as the Parsortix® PC1 System enables complete downstream analysis of the sample including whole cell imaging and proteomic analysis and full genomic and transcriptomic molecular analysis. ANGLE's commercial businesses are focusing on diagnostic products and clinical services. Diagnostic products include the Parsortix® system and associated consumables. The clinical services business is offered through ANGLE's GCP-compliant laboratories in the UK and the United States. Services include custom made assay development and clinical trial testing for pharma. Over 90 peer-reviewed publications have demonstrated the performance of the Parsortix system. For more information, visit This information is provided by Reach, the non-regulatory press release distribution service of RNS, part of the London Stock Exchange. Terms and conditions relating to the use and distribution of this information may apply. For further information, please contact rns@lseg.com or visit . SOURCE: ANGLE plc View the original press release on accesswire.com

HANGZHOU, China, Aug. 9, 2023 /PRNewswire/ -- Lynk Pharmaceuticals Co., Ltd. (hereinafter referred to as 'Lynk Pharmaceuticals'), an innovative clinical stage company, announced positive topline data from a Phase II clinical trial of LNK01001 for the treatment of atopic dermatitis. The study was a randomized, double-blind, placebo-controlled, multicenter Phase II trial conducted in adult patients (aged 18-75) with moderate-to-severe atopic dermatitis (AD) who had previously received inadequate response to topical treatment for AD or had previously received other systemic treatment for AD. The study was led by Professor Jianzhong Zhang, Director of the Dermatology Department at Peking University People's Hospital. A total of 150 patients were enrolled in the trial. The patients were randomly divided into three trial groups at a ratio of 1:1:1, namely LNK01001 high-dose, low-dose, and placebo groups. The primary efficacy endpoint of the study was the percentage change from baseline in the eczema area and severity index (EASI) score at Week 12. Preliminary data showed that after 12 weeks of treatment, patients in both the high and low dose groups showed significant improvement, with a statistically significant difference in the percentage change from baseline in EASI score compared to the placebo group, achieving the primary endpoint. Additionally, the proportion of responders achieving EASI-75 (≥75% improvement from baseline in EASI score) and Investigator's Global Assessment (IGA) response[1] were significantly higher in both high and low dose groups of LNK01001 compared to the placebo group. Improvement of pruritus is an important indicator of quality of life for patients with atopic dermatitis, and the results of the trial showed that LNK01001 had a rapid onset of improvement in pruritus, with both the high and low dose groups of LNK01001 showing significantly better improvement in pruritus indices than the placebo group at 24 hours after dosing. In terms of safety, both high and low dose groups of LNK01001 demonstrated good overall tolerability, with comparable rates of CTC grade 2+ treatment-emergent adverse events (TEAE) and serious adverse events (SAE) compared to the placebo group. No major adverse cardiovascular events (MACE), venous thromboembolism (VTE), malignancies, or severe infections were reported. Professor Jianzhong Zhang, the principal investigator of this study, the 13th Director in the Dermatology Division of the Chinese Medical Association and Director of the Dermatology Department at Peking University People's Hospital, said, "Atopic dermatitis is a disease characterized by chronic, recurrent pruritus and polymorphic inflammatory skin lesions. Controlling itch and clearing skin lesions are the most urgent treatment needs for AD patients. The results of this study suggest that LNK01001 has the potential to provide clinical benefits to adult patients with moderate to severe AD. We will continue to conduct further research and look forward to validating the efficacy and safety of LNK01001 in Phase III clinical trials to benefit more patients." "With social development and lifestyle changes, the number of atopic dermatitis patients is increasing, with a global population of 390 million. We are pleased to see that LNK01001 has shown positive results in the treatment of atopic dermatitis", said Dr. Henry Wu, Chief Development Officer of Lynk Pharmaceuticals, "We are preparing to submit an application for the End of Phase II (EOP2)/Pre-Phase III meeting to advance the clinical development of this compound and strive to benefit more patients as soon as possible." Note: [1]: IGA response is defined as achieving an IGA score of 0 or 1 (complete or near-complete clearance of skin lesions) and an improvement of ≥2 points from baseline. About Lynk Pharmaceuticals: Lynk Pharmaceuticals, a clinical stage company, was founded in 2018 by senior drug R&D experts and executives from Pfizer, Merck, and Johnson & Johnson. Lynk Pharmaceuticals is dedicated to the discovery and development of innovative drugs for the treatment of cancer, as well as immune and inflammatory diseases. Driven by a higher goal, Lynk Pharmaceuticals aims to be a market leader to address unmet medical demands by the development of innovative therapies. To date, Lynk Pharmaceuticals has independently developed several innovative new drugs and successfully completed a number of clinical studies. For more information about Lynk Pharmaceuticals, please visit: . SOURCE Lynk Pharmaceuticals Co., Ltd.