Article
Author: LaRocque, Regina C ; Diop, Cheikh ; Dai, Wenlong ; Contreras, Vanessa ; Hodge, Robert ; Cucalon, Allison ; Johnson, Rebecca I ; Feldman, Jared ; Maciorowski, Dawid ; Ryan, Edward T ; Hudspeth, Elissa ; Honko, Anna ; Freeman, Mason W ; Maisonnasse, Pauline ; Griffiths, Anthony ; Qi, Wenbin ; Price, Brian ; Hérate, Cécile ; Chichester, Jessica A ; Pampena, M Betina ; Charles, Richelle C ; Kobayashi, Dione T ; Schmidt, Aaron G ; Kahlaoui, Nidhal ; Naninck, Thibaut ; Ryan, Aisling ; Kim, Maya ; Storm, Nadia ; Zinn, Eric ; Hauser, Blake M ; Sheridan, Abigail ; Marlin, Romain ; Lemaitre, Julien ; Bhatt, Urja ; Tsong Fang, Raphaël Ho ; Dyer, Cecilia D ; Vandenberghe, Luk H ; Knox, James J ; Yaghmour, Sam ; Zabaleta, Nerea ; Cavarelli, Mariangela ; Estelien, Reynette ; McGlynn, Marion ; Le Grand, Roger ; Li, Dan ; Michalson, Kristofer T ; Wilson, James M ; van der Werf, Sylvie ; Gallouët, Anne-Sophie ; Sanmiguel, Julio ; Chapon, Catherine ; Dereuddre-Bosquet, Nathalie ; Betts, Michael R ; Chauhan, Ruchi
The SARS-CoV-2 pandemic has affected more than 185 million people worldwide resulting in over 4 million deaths. To contain the pandemic, there is a continued need for safe vaccines that provide durable protection at low and scalable doses and can be deployed easily. Here, AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity in mouse and non-human primates following a single injection and confers complete protection from SARS-CoV-2 challenge in macaques. Peak neutralizing antibody titers are sustained at 1 year and complemented by functional memory T cell responses. The AAVCOVID vector has no relevant pre-existing immunity in humans and does not elicit cross-reactivity to common AAVs used in gene therapy. Vector genome persistence and expression wanes following injection. The single low-dose requirement, high-yield manufacturability, and 1-month stability for storage at room temperature may make this technology well suited to support effective immunization campaigns for emerging pathogens on a global scale.