Trepibutone

The voltammetric behavior of trepibutone at a pencil graphite electrode (PGE) was studied by cyclic and square-wave voltammetry in B-R buffers (pH 1.81-11).Two pairs of well-defined reversible peaks are observed at 1.06 and 1.24 V over the pH range of 1.81-6.80, which are attributed to the 1-electron transfers without proton in two different chem. forms of trepibutone, protonated and nonprotonated.The cathodic peaks correspond to a 1-electron reduction of the carbonyl of the benzoyl group, and the anodic peaks correspond to a 1-electron oxidation of its free radical.When the trepibutone concentration is higher, another reversible peak at 0.39-0.26 V is observed in the pH range of 1.81-3.00, corresponding to the transfer of two electrons and two protons, which may be due to the dimer of the trepibutone free radical.Based on the oxidation peak at 1.06 V in B-R buffer (pH 1.81), a square-wave voltammetric method is proposed for the determination of trepibutone.A linear relation was obtained from 0.24 to 10 μg mL^-1 with a detection limit of 20 ng mL^-1.The PGE exhibits the best reproducibility and highest sensitivity without any addnl. procedure for the renewal of the electrode surface.The proposed method is applied to the determination of trepibutone in pharmaceutical formulations.

“The refractory jaundice” in this paper implies the benign icteric disease which was repeatedly treated with either western or traditional Chinese medicine but gave no evidence of improvement, mainly in-cluding primary sclerosing cholangitis, intrahepatic sand-like calculi, bile duct stenosis and cholestatic hepatitis etc[1,2]. Such diseases are difficult to treat by both traditional C hinese and wes tern medicine. In this study, the cholagogic and anti-inflammatory effects of cholagogic cream, and the combined ef-fects with trepibutone on refractory jaundice were observed and evaluated by experimental pathologic and biochemical examination.