Nicomol

Aiming to verify the effect of foliar fertilizer application to soybean crops in different regions of Brazil, an experiment was carried out in soybean com. crops in the states of Parana and Mato Grosso do Sul.At both study sites, the experiments were conducted simultaneously (one area with the treatment and one without).The doses of each product were defined according to the soil anal., the developmental stage of the crop, and the possible stress period.Leaf samples were collected in the R2 stage to evaluate nutritional status and biometric factors, while the plants were collected at stage R6-R7 for the determination of growth, nutrient accumulation in grains, and productivity.The data were subjected to anal. of variance and data grouping.The nutritional status and nutrient accumulation in grains showed little difference overall; however, in 83.33% of the areas treated with foliar fertilizers, increased plant growth and productivity were observed

Marine microorganisms have been a resource for novel therapeutic drugs for decades. In addition to anticancer drugs, the drug acyclovir, derived from a marine sponge, is FDA-approved for the treatment of human herpes simplex virus-1 infections. Most alphaviruses that are infectious to terrestrial animals and humans, such as Venezuelan and eastern equine encephalitis viruses (VEEV and EEEV), lack efficient antiviral drugs and it is imperative to develop these remedies. To push the discovery and development of anti-alphavirus compounds forward, this study aimed to isolate and screen for potential antiviral compounds from cultured marine microbes originating from the marine environment. Compounds from marine microbes were of interest as they are prolific producers of bioactive compounds across the spectrum of human diseases and infections. Homoseongomycin, an actinobacteria isolated from a marine sponge displayed impressive activity against VEEV from a total of 76 marine bioactive products. The 50% effective concentration (EC₅₀) for homoseongomycin was 8.6 μM for suppressing VEEV TC-83 luciferase reporter virus replication. Homoseongomycin was non-toxic up to 50 μM and partially rescued cells from VEEV induced cell death. Homoseongomycin exhibited highly efficient antiviral activity with a reduction of VEEV infectious titers by 8 log₁₀ at 50 μM. It also inhibited EEEV replication with an EC₅₀ of 1.2 μM. Mechanism of action studies suggest that homoseongomycin affects both early and late stages of the viral life cycle. Cells treated with 25 μM of homoseongomycin had a ~90% reduction in viral entry. In comparison, later stages showed a more robust reduction in infectious titers (6 log₁₀) and VEEV extracellular viral RNA levels (4 log₁₀), but a lesser impact on intracellular viral RNA levels (1.5 log₁₀). In sum, this work demonstrates that homoseongomycin is a potential anti-VEEV and anti-EEEV compound due to its low cytotoxicity and potent antiviral activity.