Endophytic Fungus of Achyrocline satureioides: Molecular Identification, Chemical Characterization, and Cytotoxic Evaluation of its Metabolites in Human Melanoma cell line

Article

Author: Spanevello, Roselia Maria ; Pedra, Nathalia Stark ; Canuto, Kirley Marques ; Ribeiro, Paulo Riceli Vasconcelos ; Ramos-Sobrinho, Roberto ; Bona, Natália Pontes ; de Queiroz Souza, Ana Sheila ; Braganhol, Elizandra ; de Souza, Priscila Oliveira

Endophytic fungi are important sources of anticancer compounds. An endophytic fungus was isolated from the medicinal plant Achyrocline satureioides, and molecularly identified as Biscogniauxia sp. (family Xylariaceae) based on partial nucleotide sequences of the internal transcribed spacer genomic region (GenBank Accession No. ON257911). The chemical characterization and cytotoxic properties of secondary metabolites produced by Biscogniauxia sp. were evaluated in a human melanoma cell line (A375). The fungus was grown in potato-dextrose liquid medium for 25 days, and the extracted compounds were subjected to solid-phase fractionation to obtain the purified F_DCM fraction, for which the metabolites were elucidated via ultra-performance chromatography coupled to a mass spectrometer. In the present study, 17 secondary metabolites of Biscogniauxia sp., including nine polyketide derivatives, five terpenoids, and three isocoumarins, were putatively identified. This is the first study to report of the ability of Biscogniauxia sp. in the production of isocoumarin orthosporin; the terpenoids nigriterpene A and 10-xylariterpenoid; the polyketide derivatives daldinin C, 7'dechloro-5'-hydroxygriseofulvin, daldinone D, Sch-642305, curtachalasin A, cytochalasin E, epoxycytochalasins Z8, Z8 isomer, and Z17. Furthermore, this study has reported the biosynthesis of Sch-642305 by a Xylariaceae fungus for the first time. F_DCM significantly reduced the viability and proliferation of human melanoma cells at half-maximal inhibitory concentrations of 10.34 and 6.89 µg/mL, respectively, and induced late apoptosis/necrosis and cell cycle arrest in G2/M phase after 72 h of treatment. Given its ability to produce unique metabolites with promising cytotoxic effects, Biscogniauxia sp. of A. satureioides may be a reservoir of compounds with important therapeutic applications.

20 Jun 2023·Plants (Basel, Switzerland)

Effects of Salicylic Acid and Macro- and Micronutrients through Foliar and Soil Applications on the Agronomic Performance, Physiological Attributes, and Water Productivity of Wheat under Normal and Limited Irrigation in Dry Climatic Conditions.

Article

Author: El-Hendawy, Salah ; Al-Suhaibani, Nasser ; Mohammed, Nabil ; Alotaibi, Majed ; Refay, Yahya ; Alsamin, Bazel

Ensuring food security with severe shortages of freshwater and drastic changes in climatic conditions in arid countries requires the urgent development of feasible and user-friendly strategies. Relatively little is known regarding the impacts of the co-application (Co-A) of salicylic acid (SA), macronutrients (Mac), and micronutrients (Mic) through foliar (F) and soil (S) application strategies on field crops under arid and semiarid climatic conditions. A two-year field experiment was designed to compare the impacts of seven (Co-A) treatments of this strategy, including a control, F_{SA + Mic}, F_{SA + Mac}, S_SA + F_Mic, S_SA + F_{SA + Mic}, S_{SA + Mic} + F_SA, and S_{SA + Mic} + F_{Mac + Mic} on the agronomic performance, physiological attributes, and water productivity (WP) of wheat under normal (NI) and limited (LMI) irrigation conditions. The results reveal that the LMI treatment caused a significant reduction in various traits related to the growth (plant height, tiller and green leaf numbers, leaf area index, and shoot dry weight), physiology (relative water content and chlorophyll pigments), and yield components (spike length, grain weight and grain numbers per spike, thousand-grain weight, and harvest index) of wheat by 11.4-47.8%, 21.8-39.8%, and 16.4-42.3%, respectively, while WP increased by 13.3% compared to the NI treatment. The different Co-A treatments have shown a 0.2-23.7%, 3.6-26.7%, 2.3-21.6%, and 12.2-25.0% increase in various traits related to growth, physiology, yield, and WP, respectively, in comparison to the control treatment. The S_SA+ F_{SA + Mic} was determined as the best treatment that achieved the best results for all studied traits under both irrigation conditions, followed by F_{SA + Mic} and S_{SA + Mic} + F_SA under LMI in addition to F_{SA + Mac} under NI conditions. It can be concluded that the Co-A of essential plant nutrients along with SA accomplished a feasible, profitable, and easy-to-use strategy to attenuate the negative impacts of deficit irrigation stress, along with the further improvement in the growth and production of wheat under NI conditions.

01 Feb 2023·Analytical and Bioanalytical Chemistry

A catalytic hairpin assembly–based Förster resonance energy transfer sensor for ratiometric detection of ochratoxin A in food samples

Article

Author: Zhang, Hong ; Lin, Yingtong ; Zhao, Mingqin ; Chu, Wenjuan ; Luo, Zhen ; Wang, Yuli ; Hu, Jiandong ; He, Fan ; Miao, Xiangmin

Ochratoxin A (OTA) poses severe risks to the environment and human health, making the development of an accurate and sensitive analytical method for OTA detection essential. In this study, a catalytic hairpin assembly (CHA)-based Förster resonance energy transfer (FRET) aptasensor was developed to detect OTA using carbon quantum dots (CDs) and 6-carboxy-fluorescein (FAM) as dual signal readout. In the presence of OTA, the aptamer specifically interacted with OTA to release the helper DNA (HP), which could open the hairpin structure of FAM-labeled hairpin DNA 1 (H₁-FAM) modified on the surface of gold nanoparticles (AuNPs). CHA between H₁-FAM and hairpin H₂ labeled with CDs (H₂-CDs) can release HP for the next cycle, resulting in the occurrence of FRET with CDs as the energy donor and FAM as the energy acceptor. According to the ratio of F_CDs/F_FAM, the proposed aptasensor showed a wide linear range from 5.0 pg/mL to 3.0 ng/mL and a low detection limit of 1.5 pg/mL for OTA detection. Moreover, satisfactory results were obtained for OTA detection in rice, suggesting the potential application of this sensor in food safety analysis.

100 Deals associated with Recombinant human histone H1.3(SymBio Pharmaceuticals)

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Indication	Highest Phase	Country/Location	Organization	Date
Acute Myeloid Leukemia	Phase 2	-	Xenetic Biosciences, Inc.	-
Acute Myeloid Leukemia	Phase 2	-	Pharmsynthez PJSC	-
Acute Myeloid Leukemia	Phase 2	-	Dana-Farber Cancer Institute, Inc.	-
Acute Myeloid Leukemia	Phase 2	-	SymBio Pharmaceuticals Ltd.	-
Non-Hodgkin Lymphoma	Phase 2	-	Pharmsynthez PJSC	-
Non-Hodgkin Lymphoma	Phase 2	-	Xenetic Biosciences, Inc.	-
Non-Hodgkin Lymphoma	Phase 2	-	Dana-Farber Cancer Institute, Inc.	-
Acute Myeloid Leukemia	Preclinical	RU	-	-
Acute Myeloid Leukemia	Preclinical	DE	-	-
Non-Hodgkin Lymphoma	Preclinical	RU	-	-

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Study	Phase	Population	Analyzed Enrollment	Group	Results	Evaluation	Publication Date


ASCO2009	Phase 1	HR-positive/HER2-low Solid Tumors	74	FOLFIRI + F	(mzranfhfxl) = arlpgkpyhi ewzmsihqih (cfqgedwxpe )	-	20 May 2009
ASCO2006	Not Applicable	Recurrent Prostate Carcinoma	26	CAB+F	mszxnyudlb(alcbtrsdwv) = uxuksjuawc rbzpncrfvo (tingxedecf ) View more	-	20 Jun 2006
ASCO2006	Not Applicable	Recurrent Prostate Carcinoma	26	ZA	mszxnyudlb(alcbtrsdwv) = wwqzwyrczu rbzpncrfvo (tingxedecf ) View more	-	20 Jun 2006

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