Acidic Exo-Polysaccharide Obtained from Bacillus sp. NRC5 Attenuates Testosterone-DMBA-Induced Prostate Cancer in Rats via Inhibition of 5 α-Reductase and Na+/K+ ATPase Activity Mechanisms

Article

Author: Ibrahim, Abeer Y ; Asker, Mohsen S ; Mahmoud, Manal G ; El-Newary, Samah A ; Youness, Eman R

AbstractBacillus sp. NRC5 is a new strain that grows in Egyptian beaches. This strain produces acidic exo-polysaccharide that have excellent antioxidant, anti-inflammatory and anti-tumor properties. The current study aimed to introduce a new natural product feasible for prostate cancer therapies. The anti-prostate cancer of acidic exo-polysaccharide produced from marine Bacillus sp. NRC5 (EBPS) was determined using 7,12-dimethylbenz-(a)-anthracene; DMBA-induced prostate cancer in male Sprague Dawley rats. Rats were subcutaneously injected with testosterone (3 mg/kg/day for 3 months) and a single dose of DMBA (65 mg/kg) for induction of prostate cancer. EBPS was administrated orally at dose 200 mg/kg/day for 3 months. To study protective effect of EBPS, animals received EBPS before cancer induction, meanwhile in therapeutic effect animals received EBPS after cancer induction. EBPS debug oxidative stress and inflammatory conditions associated with prostate cancer. EBPS either protective or therapeutic material considerably reduced cancer growth rate-limiting enzyme—i.e., 5-α-reductase (46.89 ± 1.72 and 44.86 ± 2.56 µg Eq/mL) and Na+/K+ ATPase (0.44 ± 0.03 and 0.42 ± 0.02 µg Eq/mL), compared to cancer control (69.68 ± 3.46 µg Eq/mL). In addition, both cancer biomarkers—i.e., prostate-specific antigen and carcinoembryonic antigen were significantly lowered as evidence of the ability of EBPS to protect and treat prostate cancer in chemically induced rats. EBPS showed protective and therapeutic efficacy on testosterone–DMBA-induced prostate cancer rats with a good safety margin. This study may go to clinical trials after a repeated study on another type of small experimental animal, their offspring, and one big experimental animal.

01 Dec 2022·Food Research International

Natural variation on free, esterified, glycosylated and insoluble-bound phenolics of Rubus chingii Hu: Correlation between phenolic constituents and antioxidant activities

Article

Author: Li, Chang ; Li, Bin ; Zhong, Jiahui ; Wang, Yu ; Dong, Ruihong ; Xie, Yujie ; Chen, Yi ; Tian, Jinlong ; Yu, Qiang ; Xie, Jianhua

This study sought to determine how the variation of soluble free phenolics (SFPs), soluble-bound (including esterified-bound phenolics (EBPs), glycosylated-bound phenolics (GBPs)) and insoluble-bound phenolics (ISBPs) affected the antioxidant properties (radical scavenging activities and oxidative damage caused by H₂O₂ in RAW264.7 cell) of Rubus chingii Hu during different maturity stages. Our result demonstrated that the young fruit stage could be considered as a proper harvesting period for Rubus chingii Hu to obtain high antioxidant activities. Distinct tendencies about abundance were found in four phenolic forms and followed the order: SFPs ≈ EBPs > GBPs ≈ IBPs, and antioxidant activities correlation: EBPs > GBPs > SFPs. Furthermore, total phenolic content, total flavonoid content and phenolics such as kaempferol-3-O-rutinoside, tiliroside and ellagic acid have a substantial correlation with antioxidant capacity. This study may supply a theoretical foundation for the utilization and exploitation of Rubus chingii Hu in different maturity periods.

01 Sep 2018·International Journal of Biological MacromoleculesQ1 · CHEMISTRY

Optimization, antioxidant potential, modulatory effect and anti-apoptotic action in of Euphorbia bivonae polysaccharides on hydrogen peroxide-induced toxicity in human embryonic kidney cells HEK293

Q1 · CHEMISTRY

Article

Author: Athmouni, Khaled ; El Feki, Abdelfattah ; Ayadi, Habib ; Belhaj, Dalel

For the first time, we have determined the effect of solvent, liquid-solid ratio and extraction time on polysaccharides yield was evaluated using a full factorial design (2³). In this present investigation, a total of 7 molecules were determined in this species. In our analysis saccharose was the dominant monosaccharides. Arabinose, pyranose, fructose, glucose, inositol, saccharose and trehalose found in E. bivonae. The results of the in vitro antioxidant assay showed that the EBPS have higher antioxidant capacity. Accordingly, the HEK293 cells pre-treated with EBPS compounds (100 μg·mL^-1) enhanced cell viability against H₂O₂ exposure. Our results revealed that H₂O₂-exposure induced a significant increase in intracellular ROS and lipid peroxidation in HEK293 cells. Additionally, the H₂O₂-induced alteration in HEK293 cells morphology, was ameliorated by EBPS treatment. In addition, EPBS pre-treated cells significantly enhanced the activities of HEK293 cells antioxidant status (SOD, CAT, GPx and GSH) that were decreased after hydrogen peroxide exposure.

100 Deals associated with Ebastine/Pseudoephedrine Hydrochloride

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