The purpose of this preliminary study was to discriminate the chemical fingerprints of Protected Designation of Origin (PDO) Grana Padano cheeses from non-PDO "Grana-type" cheeses by means of an untargeted metabolomic approach based on ultra-high-pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometer (UHPLC/QTOF-MS). Hierarchical cluster analysis and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) allowed discriminating PDO vs. non-PDO cheeses. Lipids (fatty acids and their derivatives, phospholipids and monoacylglycerols), amino acids and oligopeptides, together with plant-derived compounds were the markers having the highest discrimination potential. It can be postulated that Grana Padano value chain, as strictly defined in the PDO production specification rules, can drive the biochemical processes involved in cheese making and ripening in a distinct manner, thus leaving a defined chemical signature on the final product. These preliminary findings provide the basis for further authenticity studies, aiming to protect the designation of origin of PDO Grana Padano cheese by applying a comprehensive foodomics-based approach.

01 Mar 2006·Journal of Pharmaceutical and Biomedical AnalysisQ3 · MEDICINE

Degradation chemistry of a Vitamin D analogue (ecalcidene) investigated by HPLC–MS, HPLC–NMR and chemical derivatization

Q3 · MEDICINE

Article

Author: Richard Dunphy ; Sundara Katugam Srinivasetty Setty ; Fa Zhang ; Brigitte Segmuller ; Robert Henry Hesse ; Mathews Nunes

Ecalcidene (1-[(1alpha,3beta,5Z,7E,20S)-1,3-dihydroxy-24-oxo-9,10-secochola-5,7,10(19)-trien-24-yl]-piperidine) is a new 1-hydroxyvitamin D analogue. In this report, the thermal degradation, acid induced degradation and iodine induced degradation of ecalcidene were investigated using HPLC-MS, HPLC-NMR and chemical derivatization. In solution ecalcidene was thermally and reversibly transformed to a pre-Vitamin D type isomer 1 which subsequently produced the dehydrated pyrocalciferol and isopyrocalciferol type isomers 2 and 3 by cyclization and dehydration at elevated temperatures. Acidic conditions resulted in the formation of a novel C9-hydroxylated isomer 4 of ecalcidene, possibly via a tachysterol type intermediate, followed by the acid facilitated nucleophilic addition of water. In the presence of iodine, cis/trans isomerization of both ecalcidene and its pre-Vitamin D type isomer 1 occurred. The results may shed light on the stability and metabolism of ecalcidene, provide useful information for its potential pharmaceutical development, and enrich the knowledge of Vitamin D chemistry.

Investigation of degradation products and extractables in developing topical OTC (over the counter) and NCE (new chemical entity) consumer healthcare medication products

Author: Zhang, Fa

Degradation product identification, degradation pathway investigation, drug-formulation ingredient interaction, and extractables studies for NEC evaluation and marketed topical OTC product assessment are discussed.For marketed products, in addition to the value in safety and quality evaluation, the degradation mechanism information could also serve as a guideline for optimization of formulation, packaging, manufacturing, and storage condition.The power of a strategic combination of HPLC-MS with other novel anal. technologies such as HPLC-UV, HPLC-NMR, GC-MS, off-line NMR, and traditional semipreparative HPLC, chem. derivatization, and classic organic synthesis for structural identification was demonstrated.Oxidatively induced coupling of miconazole nitrate with BHT in a topical ointment, extractables or leachables from packaging including rubber closures of a prefilled semisolid drug applicator into drug products, novel degradation products and pathways of vitamin D and its analogs, thermal degradation of ecalcidene, and reductive degradation of a 1,2,4-thiadiazolium derivative, 2,3-bis(2-methoxyphenyl)-5-phenylamino-[1,2,4]thiadiazolium bromide were discussed.

100 Deals associated with Ecalcidene

R&D Status

10 top R&D records.

to view more data

Indication	Highest Phase	Country/Location	Organization	Date
Psoriasis	Discovery	-	Johnson & Johnson Consumer Products Co.	-

Clinical Result

Indication

Phase

Evaluation

View All Results

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Deal

Boost your decision using our deal data.

view full example data

Core Patent

Boost your research with our Core Patent data.

view full example data

Clinical Trial

Identify the latest clinical trials across global registries.

view full example data

Approval

Accelerate your research with the latest regulatory approval information.

view full example data

Regulation

Understand key drug designations in just a few clicks with Synapse.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis


No Data

Ecalcidene

Overview

Basic Info

Structure/Sequence

Related

R&D Status

Clinical Result

Translational Medicine

Deal

Core Patent

Clinical Trial

Approval

Regulation