Integration of physiology, genomics and microbiomics analyses reveal the biodegradation mechanism of petroleum hydrocarbons by Medicago sativa L. and growth-promoting bacterium Rhodococcus erythropolis KB1

Article

Author: Leng, Feifan ; Luo, Wen ; Wang, Yonggang ; Guo, Xiaopeng ; Zhuang, Yan ; Zhu, Ning ; Lei, Tianzhu ; Chen, Jixiang ; Sun, Shangchen

Despite the effectiveness of microbial-phytoremediation for remediating total petroleum hydrocarbons (TPH)-contaminated soil, the underlying mechanisms remain elusive. This study investigated the whole-genome and biological activity of Rhodococcus erythropolis KB1, revealing its plant growth promotion (PGP), TPH degradation, and stress resistance capabilities. Phytoremediation (using alfalfa) and plant-microbial remediation (using alfalfa and KB1) were employed to degrade TPH. The highest TPH degradation rate, reaching 95%, was observed with plant-microbial remediation. This is attributed to KB1's ability to promote alfalfa growth, induce the release of signaling molecules to activate plant antioxidant enzymes, actively recruit TPH-degrading bacteria (e.g., Sphingomonas, Pseudomonas, C1-B045), and increase soil nitrogen and phosphorus levels, thereby accelerating TPH degradation by both plants and microorganisms. This study demonstrates that R. erythropolis KB1 holds great potential for enhancing the remediation of TPH-contaminated soil through its multifaceted mechanisms, particularly in plant-microbial remediation strategies, providing valuable theoretical support for the application of this technology.

15 Apr 2024·Internal Medicine

Acute Pneumonitis Caused by Inhalation of Kakkonto Granules

Article

Author: Yatera, Kazuhiro ; Endo, Miyu ; Kanda, Hideki ; Yamasaki, Kei

01 Mar 2024·Journal of Biological Chemistry

Scanning mutagenesis identifies residues that improve the long-term stability and insecticidal activity of cyclotide kalata B1

Article

Author: Huang, Yen-Hua ; Jiang, Zhihao ; Yap, Kuok ; Wang, Conan K ; Du, Qingdan ; Craik, David J ; Kaas, Quentin ; Bigot, Aurélien

Cyclotides are plant-derived disulfide-rich cyclic peptides that have a natural function in plant defense and potential for use as agricultural pesticides. Because of their highly constrained topology, they are highly resistant to thermal, chemical, or enzymatic degradation. However, the stability of cyclotides at alkaline pH for incubation times of longer than a few days is poorly studied but important since these conditions could be encountered in the environment, during storage or field application as insecticides. In this study, kalata B1 (kB1), the prototypical cyclotide, was engineered to improve its long-term stability and retain its insecticidal activity via point mutations. We found that substituting either Asn29 or Gly1 to lysine or leucine increased the stability of kB1 by twofold when incubated in an alkaline buffer (pH = 9.0) for 7 days, while retaining its insecticidal activity. In addition, when Gly1 was replaced with lysine or leucine, the mutants could be cyclized using an asparaginyl endopeptidase, in vitro with a yield of ∼90% within 5 min. These results demonstrate the potential to manufacture kB1 mutants with increased stability and insecticidal activity recombinantly or in planta. Overall, the discovery of mutants of kB1 that have enhanced stability could be useful in leading to longer term activity in the field as bioinsecticides.

100 Deals associated with Gegen Decoction

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Indication	Country/Location	Organization	Date
Nasal Obstruction	Japan	Ohsugi Pharmaceutical Co., Ltd.	22 Aug 1986
Rhinitis	Japan	Ohsugi Pharmaceutical Co., Ltd.	22 Aug 1986
Adenotonsillitis	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Brachial Plexus Neuritis	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Conjunctivitis	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Empyema	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Mastitis	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Muscle Spasticity	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Otitis Media	Japan	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Common Cold	Japan	Kracie Pharmaceutical Ltd.	24 Jun 1986
Headache	Japan	Kracie Pharmaceutical Ltd.	24 Jun 1986

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