Author: Wu, Wenlin ; Huang, Xia ; Liu, Shiyao ; Chen, Xiaoqi ; Zhou, Yan ; Wan, Yuping ; Xia, Bing ; Guo, Tianrong ; Shi, Peiyu ; Xiao, Quanwei ; Zhang, Min

Currently, there is concern about food products to which new psychoactive substances (NPS) have been added illegally. However, rapid, high-sensitivity, and high-throughput screening methods for such harmful substances have not been reported yet. In this study, a high-throughput non-targeted screening strategy was developed based on UHPLC-Q/Orbitrap. First, optimized pretreatment methods for typical high-risk matrices were established. Second, an accurate, efficient, and scalable high resolution mass spectrometry database containing 206 NPS and related compounds was constructed. The fragmentation pathways of three different types of fentanyl-related compounds were then systematically investigated to enhance compound identification. Additionally, a non-targeted screening strategy was developed using warning ions derived from 24 fentanyl-related compounds, streamlining the identification of NPS. This strategy was applied to 100 food samples with possible illegal additives and demonstrated its effectiveness. In summary, this study provides robust technical support for regulatory authorities in combating the illegal addition of food products.

01 Jan 2025·Food Chemistry

Dual-functional probe-based multi-signal immunosensor platform for tropane alkaloids: Verification and evaluation

Article

Author: Jiang, Haiyang ; Wang, Zile ; Zhang, Shuai ; Han, Ke ; Feng, Zhiyue ; Wang, Sihan ; Sun, Boyan

This study aims to realise rapid detecting of tropane alkaloids (TAs) in food. For this purpose, a broad-spectrum single-chain fragment variable was fused with horseradish peroxidase to create an antibody-enzyme complex (AEC) with antigen recognition and catalytic activity. A multi-signal immunosensor platform based on AEC in the direct competitive reaction mode was constructed using 3,3',5,5'-tetramethylbenzidine and 10-acetyl-3,7-dihydroxyphenoxazine as substrates. The sensitivity of TAs in the immunosensor platform ranged from 0.25 μg/kg to 7912.46 μg/kg. Honey was selected as a representative food sample, and the limit of detection of TAs in honey ranged from 0.02 μg/kg to 409.11 μg/kg, with a recovery rate of 65.7 %-117.1 % and a coefficient of variation less than 21.4 %. Results showed that the immunosensor platform possesses satisfactory accuracy and precision, which highlights its potential for practical applications and its suitability as an ideal tool for rapid screening of TAs in food.

01 Apr 2023·Food Research International

A proteomic study of “Coppa Piacentina”: A typical Italian dry-cured Salami

Article

Author: Tremonte, Patrizio ; Coppola, Raffaele ; la Gatta, Barbara ; Rutigliano, Mariacinzia ; Trani, Antonio ; Dilucia, Flavia ; Spadaccino, Giuseppina ; Di Luccia, Aldo ; Loizzo, Pasqua

Coppa Piacentina is considered a peculiar dry cured salami, since it is manufactured by the entire neck muscles stuffed and matured in natural casings, the same as dry cured ham and fermented dry cured sausages. In this work the proteolysis of external and internal portions was investigated by a proteomic approach and by amino acids analysis. Samples of "Coppa Piacentina" were analyzed at 0 days and after 5 and 8 months of ripening through mono- and two-dimensional gel electrophoresis. Image analysis of 2D electrophoretic maps indicated a more intense enzyme activity on the external part, mainly due to endogenous enzymes. They favored, respectively, myofibrillar or sarcoplasmic proteins at 5 or 8 months of ripening. Free amino acids determination proved that lysine and glutamic acid were the most represented ones, followed by a free amino acids sequence like that of dry cured ham. The peculiarities of "Coppa Piacentina" were characterized by a slow proteolysis, due to sacking and binding of the whole cut of the pork neck.

100 Deals associated with Atropine Methobromide

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KEGG	Wiki	ATC	Drug Bank
-	Atropine Methobromide	A03BA01 S01FA01	-

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Indication	Country/Location	Organization	Date
Colitis	China	Shanghai Fudan Forward Pharmaceutical Co. Ltd.	-
Diarrhea	China	Shanghai Fudan Forward Pharmaceutical Co. Ltd.	-
Duodenal Ulcer	China	Shanghai Fudan Forward Pharmaceutical Co. Ltd.	-
Gastritis	China	Shanghai Fudan Forward Pharmaceutical Co. Ltd.	-
Stomach Ulcer	China	Shanghai Fudan Forward Pharmaceutical Co. Ltd.	-

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