Human hair is a filament rich in protein that grows from the follicles present in the scalp.It is considered to be a notable biomaterial consisting of β-keratin, which shows excellent catalytic activity in organic transformation.Keratin is a family of structural fibrous proteins rich in cysteine, which are abundantly present in human hair.Based on the template synthesis strategy, human-hair Keratin was used as a platform template to synthesize metal oxide nanoparticles.Here the aim was to synthesis hair protein supported CuO nanoparticles (HHP/CuONPs) and study their catalytic applications.Protein is extracted from the hair sample using the Shindai method.The HHP/CuONPs sample has been scrutinised using various characterization techniques such as UV, FT-IR, SEM, TEM, EDX, Raman, XPS, and zeta potential.The synthesized HHP/CuONPs show photo-catalytic efficiency against colored organic pollutants, 2,4-Dinitrophenol (DNP), and amaranth dye.The degradation level has been found to be 96% in DNP and 94% in amaranth dye.Optimization and reusability of hair protein-mediated copper oxide have been tested under various conditions.