Method for determination of chlorogenic acid in Yinhuang granules with HPLC was established.The conditions were as follow: μBondapak C18 (4.6 × 150 mm, 10 μm) column, acetonitrile-0.4% phosphoric acid (13:87) as mobile phase, flow rate 1.0 mL/min, column temperature room temperature and detection wavelength 327 nm.It's linear when the concentration of chlorogenic acid was between 3.98-59.70 μg/mL (γ = 0.9999), the mean recovery was 102.8% (n = 5), and RSD was 10.03%.The method is accurate, reproducible, simple, and easy to operate, which can be used in quality control.