Abstract Introduction Urinary kryptopyrroles (UKP), or the ‘Mauve factor’, was first described in the early 1960s with an observed association with psychiatric illness. Since this time, there has been growing interest in the clinical importance of UKP testing for a range of clinical conditions. However, there are substantive gaps in the available evidence to inform appropriate application and interpretation of UKP tests. With this in mind, this study describes the observed health conditions, treatments and diagnostic application of UKP by clinicians using it within their clinical practice. Methods Observational cross-sectional self-report online survey of Australian clinicians, recruited through a company that provides functional testing (including UKP analysis), who identify as having used UKP testing within their clinical practice. The survey collected data on participant demography and characteristics of their clinical practice, their approach to the diagnosis and interventions used to clinically manage elevated UKP (eUKP), and the observed clinical importance of eUKP for a range of health conditions. Results The survey was completed by 86 respondents. The majority of participants used nutritional supplements (76.7%), dietary therapy (60.5%) and lifestyle changes (58.1%) to clinically manage eUKP. Nervous system (86.0%) and gastrointestinal (64.0%) conditions were most commonly observed by respondents to be linked to eUKP. Anxiety was observed by 91.9% of respondents to be associated with eUKP and was also the condition most commonly described as markedly improving following treatment of eUKP. A number of factors were reported to be associated with eUKP by the majority of respondents including mood swings (100%), social withdrawal (92.5%), compulsive behaviour (82.4%) and emotional eating (77.8%). Implications Given the substantive gaps in evidence associated with UKP testing, this data may provide direction for researchers to design projects that reflect UKP testing as it is used in grass-roots clinical practice.