catalase

Aging and related diseases are associated with alterations in oxidative status and low-grade inflammation, as well as a decreased endoplasmic reticulum (ER) unfolded protein response (UPR). UPR is a functional mechanism by which cells attempt to protect themselves against ER stress. Researchers analyzed these proteins in peripheral blood mononuclear cells of elderly subjects and used computer simulation to predict the key proteins associated with these biomolecules underlying physiological adaptations to exercise. They collected blood samples about five to six days before and after the training period and analyzed various oxidative stress biomarkers in peripheral blood mononuclear cells. The study takes research one step further in helping to elucidate the benefits of exercise in this population. Aging involves a balance between oxidants and antioxidants, low-grade inflammation and a protein response that occurs at the cellular level, which is responsible for many health disorders. Exercise has been shown to regulate the inflammatory response, balance oxidants such as free radicals that build up in the cells and damage DNA; and ameliorate the process by which cells protect themselves against these stressors. Furthermore, resistance training in older adults is recommended to help maintain muscle, flexibility and balance. Aging and related diseases are associated with alterations in oxidative status and low-grade inflammation, as well as a decreased endoplasmic reticulum (ER) unfolded protein response (UPR). UPR is a functional mechanism by which cells attempt to protect themselves against ER stress, resulting from the accumulation of the unfolded/misfolded proteins. One group of proteins associated with the aging process is the mitochondrial heat shock protein 60 (HSP60), which has been demonstrated to play a protective role in the ability for cells to remain active and healthy. Currently, there is limited research investigating the effects of resistance training in older adults on the expression of HSP60 and Klotho, a gene involved in the aging process in mammals. A new study by researchers at Florida Atlantic University, in collaboration with the University of León in Spain, examined whether an eight-week resistance training program would modulate the oxidative status, the UPR activation and key inflammatory pathways as well as their relationships with HSP60 and Klotho proteins. For the study, researchers analyzed these proteins in peripheral blood mononuclear cells of elderly subjects. In addition, they utilized computer simulation to predict the key proteins associated with these biomolecules underlying physiological adaptations to exercise. They collected blood samples approximately five to six days before and after the training period and just before training intervention in young subjects who were included for basal assessments. Researchers also analyzed various oxidative stress biomarkers in peripheral blood mononuclear cells. Results of the study, published in the journal Antioxidants, demonstrated that the levels of the inflammatory proteins (pIRAK1, TLR4, and TRAF6), as well as different markers of the redox balance (catalase, GSH, LP, NRF2, PC, ROS, SOD1, and SOD2) remained unchanged with training. Importantly, untrained elderly subjects showed a significant reduction in pIRE1/IRE1 ratio when compared to trained elderly subjects. Such a finding was further confirmed by a gene ontology analysis, showing that endoplasmic reticulum stress is a key mechanism modulated by IRE1. Additionally, analysis did not show the training effect on the expression of HSP60 and Klotho or their relationships with other outcome variables. Although elderly male and female subjects were included in the training program, researchers did not find any sex effects in the study. These findings might partially support the modulatory effect of resistance training on the endoplasmic reticulum in the elderly. "Regular physical activity is suggested to be an effective intervention in improving age-related diseases such as osteoporosis, sarcopenia or muscle loss and dynapenia or loss of muscle strength, cardiovascular diseases, and type 2 diabetes," said Chun-Jung "Phil" Huang, Ph.D., co-author and a professor in the Department of Exercise Science and Health Promotion within FAU's Charles E. Schmidt College of Science. "Although the beneficial effects of regular physical exercise to alleviate inflammation and oxidative stress are well-established, the processes of these physiological adaptations with regard to protein folding or UPR remains to be explored. That is why we used a systems biology approach for our study." The resistance training protocol for the study consisted of 16 sessions over eight weeks (two sessions per week), with a minimum of 48 hours between sessions. The participants started with a 10-minute warm-up on a cycle ergometer. Subsequently, eight different resistance exercises (leg press, ankle extension, bench press, leg extension, bicep curl, pec deck, high pulley traction, and dumbbell lateral lift) were performed using the exercise device. For each exercise, participants performed three sets of 12-8-12 repetitions. There was a two-to-three-minute rest between each repetition and a three-minute rest between each exercise. "We know how very important physical activity is for older adults and our study takes research one step further in helping to elucidate the benefits of exercise in this population," said Huang. Study co-authors are senior author Brisamar Estébanez, Ph.D.; Marta Rivera-Viloria; and José A. de Paz, M.D., all with the University of León; José E. Vargas, Ph.D., Universidad Federal do Paraná, Curtiba; and Nishant P. Visavadiay, Ph.D.; and Andy V. Kahmoui, Ph.D., both with FAU's Department of Exercise Science and Health Promotion.

Dublin, April 30, 2021 (GLOBE NEWSWIRE) -- The "Oxidative Stress Assay Market by Product (Consumable, Instruments, Kits, Services), Test Type (Indirect, Nucleic Acid, Glutathione, ROS), Technology (ELISA, Chromatography), Diseases (CVD, Oncology) End Users (Pharma & Biotech) - Global Forecasts to 2025" report has been added to ResearchAndMarkets.com's offering. The oxidative stress assay market is projected to reach USD 1,326 million by 2025 from USD 836 million in 2020, at a CAGR of 9.7%. Growth in the oxidative stress assay market can be attributed to growth in the biopharmaceutical industry and increasing public-sector funding for academic research. Other important factors aiding the growth of this market include technological advancements like HCS for drug discovery, increasing R&D expenditure in biotechnology and pharmaceutical industries, and government support for pharmaceutical and biotechnology industries. On the other hand, the high cost of instruments and shortage of trained and qualified personnel are the major factors restraining the growth of this market. The consumables segment accounted for the largest market share in 2020. Based on products, the oxidative stress assay market is segmented into consumables, instruments, and software and services. Consumables is the largest and fastest-growing segment in 2020. This large share and high growth can be attributed the recurring requirement of consumables as compared to instruments which is the key factor driving the growth of this segment. The cardiovascular diseases segment will continue to dominate the oxidative stress assay market during the forecast period. Based on disease type, the oxidative stress assays market is segmented into cardiovascular diseases, diabetes, cancer, respiratory diseases, and other diseases. The cardiovascular diseases segment accounted for the largest share of this market in 2020, primarily due to the high and growing prevalence of cardiovascular diseases across the globe. The oxidative stress assay market in the Asia Pacific region is expected to witness the highest growth rate during the forecast period. Geographically, the oxidative stress assay market is segmented into North America, Europe, Asia Pacific, and Rest of the World. The Asia Pacific market is expected to grow at the highest CAGR during the forecast period, primarily due to the reason that the Asia Pacific oxidative stress assays market offers significant growth opportunities for market players to expand their presence in this region. Emerging markets such as China and India are witnessing growth due to the large patient population and increasing healthcare expenditure. This has resulted in the increasing acceptance of advanced technologies, such as oxidative stress assays, in the Asia Pacific region. Key Topics Covered: 1 Introduction 2 Research Methodology 3 Executive Summary 4 Premium Insights4.1 Oxidative Stress Assays Market Overview4.2 North America: Oxidative Stress Assay Consumables Market (2020)4.3 Oxidative Stress Assays Market: Geographic Growth Opportunities 5 Market Overview5.1 Introduction5.2 Market Dynamics5.2.1 Drivers5.2.1.1 Rapid Growth in the Biopharmaceutical and Biotechnology Industries5.2.1.2 Growing R&D Expenditure in the Pharmaceutical & Biopharmaceutical Industries5.2.1.3 Technological Advancements Such as High-Content Screening for Drug Discovery5.2.1.4 Government Funding for Life Science Research5.2.1.5 Emergence of Label-Free Detection Technologies5.2.2 Restraints5.2.2.1 High Cost of Instruments5.2.2.2 Shortage of Trained Personnel for New and Emerging Technologies5.2.3 Opportunities5.2.3.1 Emerging Economies Present Significant Growth Opportunities5.2.3.2 Applications of Oxidative Stress Measurement in the Cosmetic Industry5.3 Porter's Five Forces Analysis5.3.1 Threat of New Entrants5.3.2 Threat of Substitutes5.3.3 Bargaining Power of Suppliers5.3.4 Bargaining Power of Buyers5.3.5 Degree of Competition5.4 Supply Chain Analysis5.5 Value Chain Analysis5.6 Ecosystem Analysis of the Oxidative Stress Assays Market5.7 COVID-19 Impact Analysis 6 Oxidative Stress Assays Market, by Product & Service6.1 Introduction6.2 Consumables6.2.1 Kits6.2.1.1 Kits Accounted for the Largest Share of the Oxidative Stress Assays Market6.2.2 Reagents6.2.2.1 Antibodies are the Most Widely Used Reagents in Oxidative Stress Assays6.3 Instruments6.3.1 Instruments Segment to Witness Lower Growth Than the Consumables Segment6.4 Services6.4.1 Services Segment to Account for a Low Share of the Market During the Forecast Period 7 Oxidative Stress Assays Market, by Technology7.1 Introduction7.2 Elisa7.2.1 Elisa Segment to Dominate the Market During the Forecast Period7.3 Flow Cytometry7.3.1 Flow Cytometry is Useful for the Measurement of Cellular Oxidative Stress7.4 Chromatography7.4.1 Gas Chromatography is the Preferred Method for the Analysis of Hne7.5 Microscopy7.5.1 Digital Microscopes with Advanced Features are Used to Evaluate Oxidative Stress7.6 High-Content Screening7.6.1 Hcs is an Emerging Technology in the Oxidative Stress Assays Market7.7 Label-Free Detection7.7.1 Label-Free Detection Segment to Witness the Highest Growth During the Forecast Period 8 Oxidative Stress Assays Market, by Test Type8.1 Introduction8.2 Indirect Assays8.2.1 Protein-Based Assays8.2.1.1 Protein-Based Assays Accounted for the Largest Share of the Indirect Assays Market8.2.2 Lipid-Based Assays8.2.2.1 The Most Commonly Used Lipid Peroxidation Assays are Mda Assays8.2.3 Nucleic Acid-Based Assays8.2.3.1 Nucleic Acid-Based Assays Accounted for the Lowest Share of the Indirect Assays Market8.3 Antioxidant Capacity Assays8.3.1 Glutathione Assays8.3.1.1 Glutathione Assays Accounted for the Largest Share of the Antioxidant Capacity Assays Market8.3.2 Ascorbic Acid Assays8.3.2.1 Ascorbic Acid Assay Kits are Based on Frasc Chemistry8.3.3 Cell-Based Exogenous Antioxidant Assays8.3.3.1 These Assays are Used for Measuring the Activity of an Exogenous Antioxidant Compound Within Adherent Cells8.4 Enzyme-Based Assays8.4.1 Catalase Activity Assays and Sod Assays are the Two Types of Enzyme-Based Assays Available in the Market8.5 Reactive Oxygen Species-Based Assays8.5.1 Ros Assay Kits Measure the Activity of Hydroxyl, Peroxyl, and Other Reactive Oxygen Species 9 Oxidative Stress Assays Market, by Disease Type9.1 Introduction9.2 Cardiovascular Diseases9.2.1 High Prevalence of Cardiovascular Diseases Will Drive Market Growth9.3 Cancer9.3.1 Rising Incidence of Cancer to Drive Market Growth9.4 Diabetes9.4.1 High and Growing Prevalence of Diabetes to Boost Market Growth9.5 Respiratory Diseases9.5.1 Rising Prevalence of Copd to Boost Market Growth9.6 Other Diseases 10 Oxidative Stress Assays Market, by End-user10.1 Introduction10.2 Pharmaceutical and Biotechnology Companies10.2.1 Pharmaceutical and Biotechnology Companies are the Largest End-users of Oxidative Stress Assays10.3 Academic Research Institutes10.3.1 Strengthening of Drug Discovery Research Programs to Drive Market Growth10.4 Clinical Laboratories10.4.1 Oxidative Stress Assays are Used in Preclinical Biological Testing10.5 Contract Research Organizations10.5.1 Outsourcing of Pharmaceutical R&D Activities to Drive Market Growth10.6 Cosmetic Industry10.6.1 Adoption of Oxidative Stress Assays is Expected to Increase in the Cosmetic Industry 11 Oxidative Stress Assays Market, by Region11.1 Introduction11.2 North America11.3 Europe11.4 Asia-Pacific11.5 Rest of the World 12 Competitive Landscape12.1 Overview12.2 Market Share Analysis12.2.1 Introduction12.3 Company Evaluation Matrix12.3.1 Stars12.3.2 Emerging Leaders12.3.3 Pervasive Players12.3.4 Participants12.4 Competitive Situations and Trends12.4.1 Key Partnerships, Agreements, and Collaborations (2017-2020)12.4.2 Key Product Launches (2017-2020)12.4.3 Key Acquisitions (2017-2020)12.4.4 Key Expansions (2017-2020) 13 Company Profiles13.1 Major Players13.1.1 Abcam plc13.1.2 Enzo Biochem, Inc.13.1.3 Merck KGaA13.1.4 Qiagen N.V.13.1.5 Thermo Fisher Scientific, Inc.13.1.6 Ams Biotechnology (Europe) Ltd.13.1.7 Biovision, Inc.13.1.8 Cell Biolabs, Inc.13.1.9 Oxford Biomedical Research13.1.10 Cayman Chemical13.2 Other Players13.2.1 Eagle Biosciences, Inc.13.2.2 Arbor Assays13.2.3 Hycult Biotech13.2.4 Kamiya Biomedical Company13.2.5 Mega Tip San.Tic.Ltd.Sti.13.2.6 Promega Corporation13.2.7 Callegari Srl13.2.8 Immunochemistry Technologies, LLC13.2.9 Genox Corporation13.2.10 Toxys B.V. 14 Appendix14.1 Discussion Guide14.2 Knowledge Store: The Subscription Portal14.3 Available Customizations For more information about this report visit

Dublin, April 30, 2021 (GLOBE NEWSWIRE) -- The "Oxidative Stress Assay Market by Product (Consumable, Instruments, Kits, Services), Test Type (Indirect, Nucleic Acid, Glutathione, ROS), Technology (ELISA, Chromatography), Diseases (CVD, Oncology) End Users (Pharma & Biotech) - Global Forecasts to 2025" report has been added to ResearchAndMarkets.com's offering. The oxidative stress assay market is projected to reach USD 1,326 million by 2025 from USD 836 million in 2020, at a CAGR of 9.7%. Growth in the oxidative stress assay market can be attributed to growth in the biopharmaceutical industry and increasing public-sector funding for academic research. Other important factors aiding the growth of this market include technological advancements like HCS for drug discovery, increasing R&D expenditure in biotechnology and pharmaceutical industries, and government support for pharmaceutical and biotechnology industries. On the other hand, the high cost of instruments and shortage of trained and qualified personnel are the major factors restraining the growth of this market. The consumables segment accounted for the largest market share in 2020. Based on products, the oxidative stress assay market is segmented into consumables, instruments, and software and services. Consumables is the largest and fastest-growing segment in 2020. This large share and high growth can be attributed the recurring requirement of consumables as compared to instruments which is the key factor driving the growth of this segment. The cardiovascular diseases segment will continue to dominate the oxidative stress assay market during the forecast period. Based on disease type, the oxidative stress assays market is segmented into cardiovascular diseases, diabetes, cancer, respiratory diseases, and other diseases. The cardiovascular diseases segment accounted for the largest share of this market in 2020, primarily due to the high and growing prevalence of cardiovascular diseases across the globe. The oxidative stress assay market in the Asia Pacific region is expected to witness the highest growth rate during the forecast period. Geographically, the oxidative stress assay market is segmented into North America, Europe, Asia Pacific, and Rest of the World. The Asia Pacific market is expected to grow at the highest CAGR during the forecast period, primarily due to the reason that the Asia Pacific oxidative stress assays market offers significant growth opportunities for market players to expand their presence in this region. Emerging markets such as China and India are witnessing growth due to the large patient population and increasing healthcare expenditure. This has resulted in the increasing acceptance of advanced technologies, such as oxidative stress assays, in the Asia Pacific region. Key Topics Covered: 1 Introduction 2 Research Methodology 3 Executive Summary 4 Premium Insights4.1 Oxidative Stress Assays Market Overview4.2 North America: Oxidative Stress Assay Consumables Market (2020)4.3 Oxidative Stress Assays Market: Geographic Growth Opportunities 5 Market Overview5.1 Introduction5.2 Market Dynamics5.2.1 Drivers5.2.1.1 Rapid Growth in the Biopharmaceutical and Biotechnology Industries5.2.1.2 Growing R&D Expenditure in the Pharmaceutical & Biopharmaceutical Industries5.2.1.3 Technological Advancements Such as High-Content Screening for Drug Discovery5.2.1.4 Government Funding for Life Science Research5.2.1.5 Emergence of Label-Free Detection Technologies5.2.2 Restraints5.2.2.1 High Cost of Instruments5.2.2.2 Shortage of Trained Personnel for New and Emerging Technologies5.2.3 Opportunities5.2.3.1 Emerging Economies Present Significant Growth Opportunities5.2.3.2 Applications of Oxidative Stress Measurement in the Cosmetic Industry5.3 Porter's Five Forces Analysis5.3.1 Threat of New Entrants5.3.2 Threat of Substitutes5.3.3 Bargaining Power of Suppliers5.3.4 Bargaining Power of Buyers5.3.5 Degree of Competition5.4 Supply Chain Analysis5.5 Value Chain Analysis5.6 Ecosystem Analysis of the Oxidative Stress Assays Market5.7 COVID-19 Impact Analysis 6 Oxidative Stress Assays Market, by Product & Service6.1 Introduction6.2 Consumables6.2.1 Kits6.2.1.1 Kits Accounted for the Largest Share of the Oxidative Stress Assays Market6.2.2 Reagents6.2.2.1 Antibodies are the Most Widely Used Reagents in Oxidative Stress Assays6.3 Instruments6.3.1 Instruments Segment to Witness Lower Growth Than the Consumables Segment6.4 Services6.4.1 Services Segment to Account for a Low Share of the Market During the Forecast Period 7 Oxidative Stress Assays Market, by Technology7.1 Introduction7.2 Elisa7.2.1 Elisa Segment to Dominate the Market During the Forecast Period7.3 Flow Cytometry7.3.1 Flow Cytometry is Useful for the Measurement of Cellular Oxidative Stress7.4 Chromatography7.4.1 Gas Chromatography is the Preferred Method for the Analysis of Hne7.5 Microscopy7.5.1 Digital Microscopes with Advanced Features are Used to Evaluate Oxidative Stress7.6 High-Content Screening7.6.1 Hcs is an Emerging Technology in the Oxidative Stress Assays Market7.7 Label-Free Detection7.7.1 Label-Free Detection Segment to Witness the Highest Growth During the Forecast Period 8 Oxidative Stress Assays Market, by Test Type8.1 Introduction8.2 Indirect Assays8.2.1 Protein-Based Assays8.2.1.1 Protein-Based Assays Accounted for the Largest Share of the Indirect Assays Market8.2.2 Lipid-Based Assays8.2.2.1 The Most Commonly Used Lipid Peroxidation Assays are Mda Assays8.2.3 Nucleic Acid-Based Assays8.2.3.1 Nucleic Acid-Based Assays Accounted for the Lowest Share of the Indirect Assays Market8.3 Antioxidant Capacity Assays8.3.1 Glutathione Assays8.3.1.1 Glutathione Assays Accounted for the Largest Share of the Antioxidant Capacity Assays Market8.3.2 Ascorbic Acid Assays8.3.2.1 Ascorbic Acid Assay Kits are Based on Frasc Chemistry8.3.3 Cell-Based Exogenous Antioxidant Assays8.3.3.1 These Assays are Used for Measuring the Activity of an Exogenous Antioxidant Compound Within Adherent Cells8.4 Enzyme-Based Assays8.4.1 Catalase Activity Assays and Sod Assays are the Two Types of Enzyme-Based Assays Available in the Market8.5 Reactive Oxygen Species-Based Assays8.5.1 Ros Assay Kits Measure the Activity of Hydroxyl, Peroxyl, and Other Reactive Oxygen Species 9 Oxidative Stress Assays Market, by Disease Type9.1 Introduction9.2 Cardiovascular Diseases9.2.1 High Prevalence of Cardiovascular Diseases Will Drive Market Growth9.3 Cancer9.3.1 Rising Incidence of Cancer to Drive Market Growth9.4 Diabetes9.4.1 High and Growing Prevalence of Diabetes to Boost Market Growth9.5 Respiratory Diseases9.5.1 Rising Prevalence of Copd to Boost Market Growth9.6 Other Diseases 10 Oxidative Stress Assays Market, by End-user10.1 Introduction10.2 Pharmaceutical and Biotechnology Companies10.2.1 Pharmaceutical and Biotechnology Companies are the Largest End-users of Oxidative Stress Assays10.3 Academic Research Institutes10.3.1 Strengthening of Drug Discovery Research Programs to Drive Market Growth10.4 Clinical Laboratories10.4.1 Oxidative Stress Assays are Used in Preclinical Biological Testing10.5 Contract Research Organizations10.5.1 Outsourcing of Pharmaceutical R&D Activities to Drive Market Growth10.6 Cosmetic Industry10.6.1 Adoption of Oxidative Stress Assays is Expected to Increase in the Cosmetic Industry 11 Oxidative Stress Assays Market, by Region11.1 Introduction11.2 North America11.3 Europe11.4 Asia-Pacific11.5 Rest of the World 12 Competitive Landscape12.1 Overview12.2 Market Share Analysis12.2.1 Introduction12.3 Company Evaluation Matrix12.3.1 Stars12.3.2 Emerging Leaders12.3.3 Pervasive Players12.3.4 Participants12.4 Competitive Situations and Trends12.4.1 Key Partnerships, Agreements, and Collaborations (2017-2020)12.4.2 Key Product Launches (2017-2020)12.4.3 Key Acquisitions (2017-2020)12.4.4 Key Expansions (2017-2020) 13 Company Profiles13.1 Major Players13.1.1 Abcam plc13.1.2 Enzo Biochem, Inc.13.1.3 Merck KGaA13.1.4 Qiagen N.V.13.1.5 Thermo Fisher Scientific, Inc.13.1.6 Ams Biotechnology (Europe) Ltd.13.1.7 Biovision, Inc.13.1.8 Cell Biolabs, Inc.13.1.9 Oxford Biomedical Research13.1.10 Cayman Chemical13.2 Other Players13.2.1 Eagle Biosciences, Inc.13.2.2 Arbor Assays13.2.3 Hycult Biotech13.2.4 Kamiya Biomedical Company13.2.5 Mega Tip San.Tic.Ltd.Sti.13.2.6 Promega Corporation13.2.7 Callegari Srl13.2.8 Immunochemistry Technologies, LLC13.2.9 Genox Corporation13.2.10 Toxys B.V. 14 Appendix14.1 Discussion Guide14.2 Knowledge Store: The Subscription Portal14.3 Available Customizations For more information about this report visit