An HPLC method was developed for the content determination of ginsenoside Rg1 in Danbie capsules.A Hypersil ODS column (4.6 mm × 150 mm, 5 μm) was adopted with acetonitrile-4% methanol solution (18: 82) as mobile phase.The flow rate was 1.5 mL/min-1, the detection wavelength was 203 nm and the column temperature was 35°.The linear range was 0.99-9.94 μg (r = 0.9999).The average recovery was 96.0%, and RSD was 2.3% (n = 5).The method is reliable, reproducible, and high precision.It can be used for the determination of ginsenoside Rg1 in Danbie capsules.