Chinese Center For Disease Control & Prevention

ClinicalTrials.gov (NCT03962816).https://clinicaltrials.gov/ct2/show/NCT03962816.

To explore the multicenter characteristics of endocrine-like phthalate esters (PAEs) in household dust and propose effective control strategies for global indoor public health. An on-site observational investigation was conducted in nine Chinese cities from 2018 to 2019. A total of 246 household dust samples were collected and analyzed for ten PAE congeners using Gas Chromatography-Mass Spectrometry (GC-MS). Questionnaires were used to gather information on building conditions, indoor behaviors, and ventilation habits. In residential dust from the nine cities, the total concentrations of the ten PAE congeners (∑PAEs) ranged from 0.921 to 29097.297 µg/g. Dicyclohexyl phthalate (DCHP) and di (2-ethylhexyl) phthalate (DEHP) were the dominant congeners in ∑PAEs. Childhood exposure to PAEs through dust ingestion was four orders of magnitude higher than through inhalation, with a carcinogenic risk of 5.47 × 10^-6 for DEHP exposure in household dust. Higher ∑PAEs concentrations were associated with higher temperature, double glazing, wall paint usage, television and computer use, and indoor plant growth. This multicenter on-site investigation confirmed PAE pollution characteristics and uncovered the inacceptable risk of daily DEHP exposure in household dust under real living conditions. Effective mitigation measures based on household-related information, residential characteristics, decoration materials, and lifestyle should be taken to build a healthy household environment.

Listeria monocytogenes (LM) is an opportunistic foodborne pathogen responsible for listeriosis in both humans and animals.The disease manifests in a variety of ways, including febrile gastroenteritis, septicemia, meningitis, and in some cases, preterm birth and spontaneous abortion.It is therefore crucial to develop effective strategies to control this bacterium.In this study, we isolated and characterized a novel Listeria phage, named LMLPA3.Morphol. and genomic analyses revealed that phage LMLPA3 belongs to the class Caudovirales, family Herelleviridae, Myovirus-like.Phage LMLPA3 demonstrated remarkable stability across a range of pH values (4-10), temperatures (4-50 °C), and high NaCl concentrations (12% w/v).A total of 68 strains, comprising nine serotypes of L. monocytogenes and five other Listeria species, were found to be susceptible to lysis by phage LMLPA3.It is noteworthy that treatment with phage LMLPA3 resulted in a significant disruption of the biofilms formed by seven different serotype strains of L. monocytogenes, in comparison to the control.Furthermore, phage LMLPA3 effectively reduced the number of L. monocytogenes cells by 4 log₁₀ CFU/mL and 2.9 log₁₀ CFU/sample, resp., in milk and on the surface of raw beef at an MOI of 10000.In light of these findings, it can be concluded that phage LMLPA3 has the potential to serve as an effective antimicrobial in the elimination of L. monocytogenes contamination in foodstuffs.