Background: Neuropathic pain (NP) is a chronic disease; Patients with NP most commonly seek treatment for primary or secondary injury of the peripheral or central nervous system. The complex pathophysiol. of NP is not yet fully elucidated, which contributes to underassessment and undertreatment. Methods: To analyze and study mol. relationships in the spinal cord in peripheral nerve induced neuropathic pain, we used SNI-induced neuropathic pain and RNA (RNA) sequencing to analyze differ entially expressed genes (DEGs). We established an SNI (shared nerve injury) model and used third-generation transcriptome sequencing technol. to analyze messenger RNA (mRNA) expression in mouse SDH (spinal dorsal horn) tissue and obtained 325 differentially expressed genes. The differentially expressed genes were further analyzed by bioinformatics anal. A protein-protein interaction (PPI) network was constructed based on the STRING database, and Cytoscape software was used for visualization. We used the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for DEGs to perform a Gene Ontol. (GO) anal. Real-time PCR (Polymerase Chain Reaction) was performed to verify the results. Results: Atf3, Sprr1a, Anxa10, Ccl7, Ccl2, Lck, and Timp1 as well as the NF-κB TNF (Tumor Necrosis Factor) and MAPK (mitogen-activated protein kinase) signalling pathways, were implicated in SNI-induced neuropathicpain. Conclusions: These findings further deepen the understanding of NP mechanisms and therapeutic targets.