Nanjing Hengdao Pharmaceutical Technology Co Ltd.

This study aims to compare and determine the optimum extraction and separation process of the total flavonoids from Scutellaria barbata.The total flavonoids from S. barbata were extracted with an L-arginine solution, methanol, Et acetate, and n-butanol.The activities and toxicity of different extracts on HepG2 and L-O2 cells were measured by MTT assay.The TLC method revealed in the sample chromatograms that the L-arginine extract showed the same patches as the chromatogram of the standard substance.The TLC spots were clear and centralized, and the R_f values of the extract of the L-arginine solution were identical with those of the Rutin and Naringin standard preparationsThe number of HPLC chromatog. peaks of the L-arginine solution extract was more than those of the other extractsIn vitro experiments indicated that at the concentration of 400 μg mL^-1, S. barbata extracted by L-arginine solution has a significant inhibitory effect on human hepatic carcinoma HepG2 cells as well as a lower toxic effect on L-O2 cells.In conclusion, L-arginine can maintain the maximum stability of flavonoids from S. barbata.In addition, the S. barbataL-arginine extracts possess favorable anti-tumor effects.Therefore, the extraction method in the present study using L-arginine can improve the clin. use and industrial production of S. barbata preparations, and also can be a reference method for the extraction of flavonoids from other plants.