The viral inactivation effectiveness in human hepatitis B Ig (HBIG) was verified by pasteurization using pseudorabies virus (PRV), Sindbis virus and human immunodeficiency virus-1 (HIV-1) as indicators.The indicator viruses were added into hepatitis B Ig (HBIG) samples at a ratio of 1:9 (volume/volume), and the mixtures were heated in 60°C water bath for 10 h then determined for virus titers, based on which the viral inactivation effectiveness was observed, and the antibody titers against hepatitis B surface antigen (HbsAg) in samples before and after inactivation were determined by RIA.After viral inactivation, the titers of PRV, Sindbis virus and HIV-1 decreased by more than 5 LgCCID50/0.1 mL, 6 LgPFU/mL and 4 Lg CCID50/0.1 mL, and the antibody titers against HBsAg in 3 batches of HBIG samples decreased by 6.5%, 6.0% and 5.8%, resp.Pasteurization was effective for inactivation of indicator viruses in HBIG.