Objective:To explore the clin. efficacy of minimally invasive nephrolithotomy on patients with urinary calculi and infection. Method:Ninety-six patients with urinary calculi and infection who admitted to the Affiliated Hospital of Jiujiang University from Jan. 2017 to Jan. 2020 were selected as the research objects and divided into the control group (n=48) and the case group (n=48) according to the random number table method. The control group was treated with soft mirror holmium laser lithotripsy, and the case group was treated with minimally invasive percutaneous nephrolithotomy. The clin. efficacy (operating time, intraoperative blood loss, hospitalization time and stone clearance rate), postoperative complications, and effects on renal function and serum inflammatory factors were compared between two groups. Result:The operation time and hospital stay in the case group were significantly shorter than those in the control group, and the amount of intraoperative blood loss was less than that in the control group, the differences were statistically significant (P<0.05). The stone clearance rate in the case group was 95.83%, which was higher than 83.33% in the control group, and the total incidence of complications was 6.25%, which was lower 20.83% of the control group, the differences were statistically significant (P<0.05). Before treatment, the levels of blood urea nitrogen (BUN), serum creatinine (Scr), uric acid (UA), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP) of two groups were compared, the differences were not statistically significant (P>0.05);after treatment, the levels of BUN, Scr, UA, IL-6, IL-8, TNF-α, CRP of two groups were lower than those before treatment, and the case group were lower than those in the control group, the differences were statistically significant (P<0.05). Conclusion:Minimally invasive percutaneous nephrolithotomy in the treatment of patients with urinary calculi and infection has a significant clin. effect, which is beneficial to improve stone removal rate, improve renal function, reduce inflammation, speed up postoperative recovery, reduce the risk of complications, and be beneficial to the long-term life of patients quality has a far-reaching impact and is worthy of clin. promotion.