Wannan Medical College

The integration of metal-organic framework (MOF) materials with magnetic cores not only serves as an effective adsorbent but also facilitates the recovery post-adsorption.In this study, we synthesized Ni@ZIF-8 composite nanotubes by introducing ZIF-8 onto the surface of Ni nanotubes, which were used as adsorbents for antibiotic removal.The Ni@ZIF-8 composite nanotubes exhibited high-efficiency and recyclable antibiotic adsorption, with the maximum adsorption capacities for tetracycline (TC) and cefixime (CFX) reaching 770.2 and 428.2 mg·g^-1, resp.The Ni nanotubes, acting as magnetic cores, facilitated magnetic recovery after adsorption.Furthermore, the introduction of ZIF-8 not only increased the surface roughness of the composite but also incorporated a significant number of functional groups.The pseudo-second-order model, Langmuir model and intra-particle diffusion models provided better fits to the isotherm and kinetic data of the two antibiotics.D. functional theory (DFT) calculations results indicate that the π-π interactions dominate the adsorption process of both TC and CFX onto ZIF-8.And the electrostatic potential (ESP) calculations and interaction anal. indicate that the Ni atom enhances the adsorption behavior through π-π interactions between the benzene ring or carbonyl group of the two antibiotics and the imidazole of ZIF-8.This research offers a novel approach for the removal of antibiotics from contaminated water and environmental remediation using magnetic core-MOF composite materials.

The abnormal viscosity of mitochondria is closely related to various cell dysfunction.Therefore, the development of effective tools that can visually monitor mitochondrial viscosity is critical for maintaining mitochondrial homeostasis.Here, based on twisted intramol. charge transfer (TICT) mechanism, we designed and synthesized a series of viscosity sensitive fluorescent probes (CTP1-CTP7) using thiophene-containing carbazole as parent unit, single bond connecting ethylene bond as viscosity sensitive "rotator" site, and pyridine salt as strong electron-withdrawing group.The fluorescence response of these probes exhibited interesting "on" and "off" behaviors in high and low viscosity environments, resp.Not only that, all these probes were highly sensitive and exclusively selective for viscosity in vitro and have excellent chem. and photostability.Biol. experiments demonstrated that the pos. charge in the probe mol. enables it to be efficiently localized in mitochondria in cells (except for CTP2).In addition, probe CTP1 displayed a high signal-to-noise ratio in fluorescence imaging and was fully capable of precise targeting of mitochondria in living cells by a wash-free procedure.More importantly, probe CTP1 has been successfully applied to visualize mitochondrial viscosity changes in a variety of drug-induced cell models and during cell death.This has practical applications for studies to elucidate the potential link between mitochondrial viscosity changes and related diseases in vivo.

Lipid droplets (LDs), as an important organelle, play a crucial role in regulating cell metabolism and growth.When their function is disrupted, they may lead to a series of diseases, which makes the monitoring of LDs the focus of researchers.In this study, relying on the lipophilic properties of triphenylamine and using its derivatives as targeting groups, we developed a series of BODIPY-based fluorescent probes (X1-X5) based on NBO structure.Probes X1, X2, and X4 all exhibited good aggregation-induced emission (AIE) performance.In addition, these probes have excellent photostability and low cytotoxicity, and have a large stokes shift (>100 nm) in H₂O.Especially, probe X4 has been successfully applied to living cell LDs imaging, providing valuable tools for the field of biol. imaging.