Objective To simultaneously determine the contents of the two target components of Yujin Dispensing Granules by the high-performance liquid chromatog.(HPLC)method, and to establish an HPLC fingerprint of the preparation Methods The chromatog. column was Welch Ultimate AQ-C_(18) column( 250 mm x 4. 6 mm, 5 μm), the mobile phase was methanol( A)-0. 1%potassium dihydrogen phosphate solution with gradient elution, the flow rate was 0. 8 m L/min, the detection wavelength was 260 nm, and the column temperature was 30 °C . The Similarity Evaluation System for Chromatog. Fingerprint of TCM(version 2012. 130723)software was used to evaluate the similarity and to determine the common peaks. Results There were three common peaks in the HPLC chromatograms of 15 batches of Curcumae Radix decoction and 4 batches of samples with similarities higher than 0. 85, the peak Iwas uridine and peak III was adenosine. The linear ranges of uridine and adenosine were 5. 1-264. 4 ng( r = 0. 999 7) and 15. 9-792. 6 ng(r = 0. 999 7), resp. The RSDs of the precision, stability and repeatability tests were less than 2. 00%( n = 6), and the average recoveries of uridine and adenosine were 93. 65%(RSD = 1. 84%, n = 6) and 93. 51%( RSD = 2. 03%, n = 6), resp. Conclusion The method is simple, accurate and reproducible, which can provide a reference for the quality evaluation of Yujin Dispensing Granules.