Yunnan Phytopharmaceutical Co. Ltd.

OBJECTIVE To compare the pharmacokinetic parameters of Xuesaitong dispersible tablets and common tablets in Beagle dogs.METHODS Using double cycle crossover trial, six Beagle dogs were treated with single oral dose of 100 mg of Xuesai- tong dispersible tablets and conventional tablets and determining the pharmacokinetic parameters of ginsenoside Rbl and Rgl in Xuesai-tong dispersible tablets and conventional tablets in Beagle dog plasma.RESULTS The ginsenoside Rbl and Rgl peak concentration of Xuesetong dispersible tablets in Beagle dog plasma was significantly higher than that of Xuesaitong tablets, the ginsenoside Rgl peak tittle of Xuesaitong dispersible tablets in Beagle dog plasma was significantly earlier than that of Xuesaitong tablets.Addnl., the ginsenoside Rbl peak time exhibited ahead of the trend, which is in line with the characteristics of rapid disintegration and absorption of preparation in vivo.CONCLUSION The plasma exposure in two preparation of ginsenoside Rbl and Rgl in Beagle dog holds fairly basic and no significant difference.But the Pmax of the main ingredients of Panax ginseng saponins Rbl and Rgl in Xuesaitong dispersed tablets, is significantly higher than that of the film coated tablets, and peak time is significantly shortened, which could promote the drug absorption.

A specific HPLC method was developed for the measurement of ginsenoside Rb₃ in Qiyeshenan tablets.Anal. was carried out on Shimadzu LC-2010A HPLC.The separation was performed on Agilent ZORBAX Bonus-RP column (5 μm, 4.6 mm × 250 mm), with acetonitrile-0.1% phosphoric acid as mobile phase, the flow rate was 1.0 mL/min^-1 and the detective wavelength was 203 nm.A good linear relationship was found in the range of 0.2-2.0 μg.The mean recovery was 98.4% (n = 15).This method is rapid, simple and accurate.

To establish an HPLC method for the determination of scutellarin in Breviscapus granules, the chromatog. column of Shimadzu VP-ODS was used.The mobile phase consisted of methanol-tetrahydrofuran-0.1% phosphoric acid (15:15:70), and the flow rate was 1.0 mL·min^-1.The detection wave-length was 335 nm.The linear range of scutellarin was 0.125-0.875 μg (r=0.9993).The average recovery of assay was 98.1% with RSD 1.3%.The HPLC method is simple and reliable for the simultaneously determination of scutellarin in Breviscapus granules.