State of Gujarat

The Al-Cu alloy is used as the main matrix material in this study, with fly ash and graphite added in.Aluminum-copper alloy composites are very popular for a number of reasons, including their good thermal conductivity and ability to work in moderate temperaturesSince graphite and fly ash particles are thin and strong, they can be used as reinforcing materials.The process of stir casting was used to make the composite.Fly ash and graphite particles were also added to the molten Al-Cu alloy in amounts of 3%, 6%, and 9%, resp.After that, the mech. properties of the composite were glanced at.These included tensile strength.SEM microstructure anal. was used to figure out how well graphite and fly ash particles should be spread out in the Al-Cu matrix.The d. of composites goes down as the amount of reinforcement materials rises.In furthermore, the addition of the reinforcing particle improves the wearability of the surface.The specimen used in the experiment is made to meet the standards set by ASTM.

MicroRNAs are small sized endogenous non-coding RNA, of ∼18-25 (nt) that regulates the expression of their target genes in plants at post transcriptional level and play very significant roles in multiple biol. processes.These ribosw itches may play the role of master regulator in important biol. processes and plant metabolic pathways.In the present study, we performed small RNA profiling to identify miRNAs present in the holy tree Aegle marmelos and their involvement in different metabolic pathways.A total of 9,192,725 (88.52%) clean reads out of 10,384,409 (100%) reads were identified with size ranging from 15- to 24 nt.Using computational tools and bioinformatics approaches we revealed around 253 conserved miRNAs accounting into 20 miRNA families and a total of 395 annotated targets were found for 6 novel miRNAs within the plant.Functional annotation revealed Homeobox-leucine zipper protein and ERF like transcription factors as targets of conserved miRNAs.The present study provides a first report on elucidation of genome-wide miRNA profile of A. marmelos which can be further explored to manipulate their expression to scale-up biosynthesis of active metabolites using metabolic engineering approaches.