Nanjing Suzhong Pharmacy Research Co., Ltd.

The methods for determination of schizandrol A in Hu Gan capsules were established.Schizandrol A was extracted by methanol soxhlet, methanol ultrasonic extraction and Et acetate reflux.Schizandrol A was determined by high-performance liquid chromatog.A Ajilent C₁₈ column (250 mm×4.6 mm) was used with the mobile phage of acetonitrile-water (50:50), flow rate was 0.1 mg/mL, and detection wavelength was at 250 nm.There were good linear relationships between the peak area and concentration (r=0.9999, n=6), and linear range was 58-2,320 μg/mL.Recovery of schizandrol A was above 98% with RSD of 1.39% (n=9).The method was accurate and could be used for quality control for the preparation

A review.According to different materials and synthetic technologies, synthetic routes of palonosetron hydrochloride are summarized in this article.

An HPLC method for determination of the content and related substances of palonosetron hydrochloride injection was established.The separation was performed on an Agilent Zorbax SB-C₁₈ column (250 mm × 4.6 mm, 5 μm) at room temperature, the mobile phase consisted of ion-pair buffer-acetonitrile (70:30) at the flow rate of 1 mL/min^-1, and the detection wavelength was 210 nm.Palonosetron hydrochloride was separated with its related substance thoroughly.There was a good linear relationship within the range of 12.00-150.01 μg/mL^-1 (r = 0.9999, n = 6).The limit of detection (LOD) was 0.5 ng; and the limit of quantitation (LOQ) was 3 ng.The average recoveries were 99.5% (RSD = 1.0%), 99.0% (RSD = 0.5%), and 99.4% (RSD = 0.9%) for high, middle and low concentrations of palonosetron hydrochloride, resp.The method is simple, rapid, and accurate and is suitable for determination of the content and related substances of palonosetron hydrochloride injection.