Ganoderma lingzhi is one of the traditional and valuable medicine and edible fungi,with high pharmacol. activity. In this study,the main chem. components and acute toxicity of Ganoderma lingzhi mycelium extract(GLME) were analyzed. The main components of GLME were protein(14.35%),hydrolyzed amino acid(6.60%),crude polysaccharide(5.74%),various minerals,unsaturated fatty acids and nucleosides. A 14-day acute toxicity experiments showed that mice given GLME by gavage administration had no mortality during the exptl. period,and there was no significant difference in their body weight,organ weight,the activity of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) compared with the control group. Therefore,GLME has potential application value for further development.