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KLK3(Prostate-specific antigen)	1

Spinal cord injury (SCI) is a devastating event which caused high mortality and morbidity. Recently, nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome has been showed to act a critical t role in the secondly injury phase of SCI. In current study, we aimed to investigate the effect and underlying molecular mechanisms of extracellular vesicles derived from epidural fat (EF)- mesenchymal stem cells (MSCs) for the treatment of SCI. Ninety-six Sprague-Dawley rats were used for current study and randomly divided into four groups: sham group, SCI group, SCI + Saline group, SCI + Extracellular vesicles group. Basso-Beattie-Bresnahan (BBB) scores was applied to evaluate the neurological functional recovery. Cresyl violet-stained was conducted evaluate the protective effect of EF-MSCs-Extracellular vesicles on lesion volume after SCI. ELISA, immunohistochemistry assay, TUNEL assay and western blotting were conducted to investigate the underlying molecular mechanisms. Our results demonstrated that the administration of EF-MSCs-Extracellular vesicles via tail vein injection improved neurological functional recovery and reduced the lesion volume after SCI. And systemic administration of EF-MSCs-Extracellular vesicles significantly inhibited NLRP3 inflammasome activation and reduced the expression of inflammatory cytokines. Additionally, the expression levels of proapoptotic protein Bax was decreased and antiapoptotic Bcl-2 was upregulated with the treatment of EF-MSCs-Extracellular vesicles after SCI. In summary, in current study, we demonstrated for the first time that the EF-MSCs-Extracellular vesicles can improve neurological functional recovery after SCI, and the underlying molecular mechanisms may partly through the inhibition of NLRP3 inflammasome activation.

01 Dec 2018·BMC BiotechnologyQ4 · ENGINEERING & TECHNOLOGY

Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis

Q4 · ENGINEERING & TECHNOLOGY

ArticleOA

Author: Chen, Huiling ; Jia, Kunzhi ; Zhang, Danping ; Yang, Qinghai ; Xie, Chengjie ; Wang, Rongzhi ; Wang, Shihua ; Ling, Sumei

BACKGROUNDHuman chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker.RESULTSThe synthesized gene of CgA coding area was cloned and expressed as fusion protein CgA-His in procaryotic system. Then the purified CgA-His protein was mixed with QuickAntibody-Mouse5W adjuvant, and injected into mice. The CgA-His protein was also used as coating antigen to determine the antiserum titer. By screening, a stable cell line named 4E5, which can generate anti-CgA monoclonal antibody (mAb), was obtained. The isotype of 4E5 mAb was IgG_2b, and the chromosome number was 102 ± 4. Anti-CgA mAb was purified from ascites fluid, and the affinity constant reached 9.23 × 10⁹ L/mol. Furthermore, the specificity of the mAb was determined with ELISA, western blot and immunohistochemistry. Results indicated that the mAb 4E5 was able to detect chromogranin A specifically and sensitively.CONCLUSIONSA sensitive and reliable method was successfully developed for rapid production of anti-CgA mAb for immunohistochemistry diagnosis in this study, and the current study also provides conclusive guidelines for preparation of mAbs and implements in immunohistochemistry diagnosis.

Schwann cells-derived Exosomes Promote Functional Recovery after Spinal Cord Injury by Promoting Angiogenesis

Author: Fu, Chun-Hui ; Huang, Jiang-Hu ; Lin, Fei-Yue

AbstractExosomes are small vesicles that contain various of miRNA, mRNA and proteins, existing in a various of tissues and cells, and plays an important role in cell-cell communication. Various cell-derived exosomes have shown a protective effect on spinal cord injury (SCI). This study investigates the neuroprotective effect of Schwann cells-derived exosomes (SCs-Exos) after SCI. We found that SCs-Exos could be uptake directly by brain-derived endothelial cells.3 (bEnd.3 cells), and promoted proliferation, migration and tube formation of bEnd.3 cells. Additionally, our results showed that two pro-angiogenesis molecules, integrin-β1 and integrin-α1 were highly expressed in SCs-Exos. Then, we used special siRNA technology to investigate the effect of Integrin-β1 in SCs-Exos induced angiogenesis on bEnd.3 cells. We observed that the pro-angiogenic effect of SCs-Exos on bEnd.3 cells were suppressed by inhibiting the expression of integrin-β1. In the SCI model, we found that SCs-Exos attenuated tissue damage and improved functional recovery after SCI. Using immunofluorescence staining, we observed that SCs-Exos treatment promoted angiogenesis after SCI, and integrin-β1 was required in this process. In conclusion, our results demonstrated that SCs-Exos promote angiogenesis after SCI, and this process is partly related to the regulation of the expression of integrin-β1.

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News (Medical) associated with Fuzhou Maixin Biotech Co., Ltd.

21 Aug 2019

·sg.news.yahoo.com

BRIEF-Beijing Strong Biotechnologies Plans To Buy Stake In Biotechnology Firm

Aug 22 (Reuters) - Beijing Strong Biotechnologies Inc : * SAYS IT AND PARTNER SIGN LETTER OF INTENT TO BUY 95.55% STAKE IN FUZHOU BIOTECHNOLOGY FIRM MXB Source text in Chinese: https://bit.ly/2ZleZqO Further company coverage: (Reporting by Hong Kong newsroom)

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100 Deals associated with Fuzhou Maixin Biotech Co., Ltd.

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100 Translational Medicine associated with Fuzhou Maixin Biotech Co., Ltd.

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Pipeline Snapshot as of 08 May 2025

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

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