Objectives The transparent surface of the eye, called the cornea, plays a crucial role in transmitting light to the retina and in protecting the eye. On its external surface, the cornea is composed of a constantly renewing multistratified epithelium. This mechanism is fueled by stem cells located in the limbus (the transition zone between the cornea and the sclera). Limbal Stem Cell Deficiency (LSCD) is characterized by a decrease or cessation of epithelial renewal and neovascularization of the cornea. Consequently, the cornea loses its integrity and transparency. This visually impairing condition is currently in a therapeutic impasse as only autologous limbal or allogeneic corneal grafts are viable options, but they pose significant risks to patients.
Studies have shown that the oral cavity contains stem cells that can be isolated, cultured, and transdifferentiated into limbal stem cells (LSCs). However, to date, these studies are limited, and no protocol has been validated. In this study, the advantage of the accessibility of the oral cavity is used to develop a protocol for differentiating cells from the oral mucosa into limbal stem cells (LSCs) for use in a future clinical trial with patients.
Methodology This prospective monocentric study will be conducted on patients from the ophthalmology department of the Montpellier University Hospital who have an indication for conjunctival reconstruction. After obtaining consent from the patients, cells from the oral wall will be sent to the tissue bank of the Montpellier University Hospital, where they will be cultured. Finally, the transdifferentiation steps will be analyzed by the "Eye" research team at the Institute of Neuroscience in Montpellier, which is collaborating on the project. The investigators have established objective factors to assess the success of the developed protocol based on the literature : (i) > 3% of stem cells in primary cultures, (ii) <10% aborted colonies, (iii) Expression of LSC markers (Pax6, Krt14, p63).

Start Date19 Jun 2024

Sponsor / Collaborator

University Hospital of Montpellier

[+1]

100 Clinical Results associated with The Institute For Neurosciences of Montpellier

0 Patents (Medical) associated with The Institute For Neurosciences of Montpellier

210

Literatures (Medical) associated with The Institute For Neurosciences of Montpellier

01 Jul 2025·Sleep Medicine

Reproduction life related Kleine-Levin syndrome

Review

Author: Billiard, Michel ; Jaussent, Isabelle

Menstrual-related Kleine-Levin syndrome is characterized by recurrent episodes of hypersomnolence exclusively associated with menstruation, occurring just before or during menses. However, the literature shows that these episodes may also be associated with other events of the reproduction life, namely menarche, ovulation, pregnancy, delivery and menopause. Altogether, we identified 33 cases of reproduction life-related Kleine-Levin syndrome in the world literature. We reviewed their clinical symptoms, compared them with those reported in 111 female cases of the classical Kleine-Levin syndrome, and reviewed available results of laboratory tests and treatments. The patterns of associations between first and subsequent hypersomnolent episodes and events of the reproduction life were extremely polymorphic. Clinically, all symptoms described in the classical Kleine-Levin syndrome were found in the reproduction life related Kleine-Levin syndrome. However, apathy and anorexia, the opposite of compulsive eating, were more frequent in the reproduction life related Kleine-Levin syndrome than in the classical female Kleine-Levin syndrome while compulsive eating and drinking were less frequent in the reproduction life related Kleine-Levin syndrome than in the classical female Kleine-Levin syndrome.

01 Mar 2025·Anesthesiology

Ultrasound-guided Transcutaneous Phrenic Nerve Stimulation in Critically Ill Patients: A New Method to Evaluate Diaphragmatic Function

Article

Author: Vonarb, Aurelie ; Carr, Julie ; Choquet, Olivier ; Capdevila, Mathieu ; De Jong, Audrey ; Belafia, Fouad ; Capdevila, Xavier ; Jaber, Samir ; Molinari, Nicolas

Background:Diaphragm dysfunction is common in intensive care unit and associated with weaning failure and mortality. The diagnosis gold standard is the transdiaphragmatic or tracheal pressure induced by magnetic phrenic nerve stimulation. However, the equipment is not commonly available and requires specific technical skills. This study aimed to evaluate ultrasound-guided transcutaneous phrenic nerve stimulation for daily bedside assessment of diaphragm function by targeted electrical phrenic nerve stimulation.Methods:This randomized crossover study compared a new method of ultrasound-guided transcutaneous electrical phrenic nerve stimulation (SONOTEPS) using a peripheral nerve stimulator, with magnetic phrenic nerve stimulation. Intensive care unit adult patients under mechanical ventilation with a Richmond Agitation–Sedation Scale score of −4 or −5 were included. Each patient received the two methods of stimulation, in a randomized order. The primary outcome was the tracheal pressure induced by stimulation.Results:This study analyzed 232 measures of tracheal pressure from 116 patients, of whom 77 presented diaphragm dysfunction (tracheal pressure less than 11 cm H2O) and 50 presented severe diaphragm dysfunction (tracheal pressure less than 8 cm H2O). The Passing–Bablok regression showed no significant differences (intercept A of −0.03 [95% CI, −0.83 to 0.52] and slope B of 0.98 [95% CI, 0.90 to 1.05]) between the SONOTEPS method and magnetic stimulation, which were positively correlated (R² = 0.639). The mean bias was −1.08 (95% CI, 5.02 to −7.18) cm H2O. The receiver operating curves showed an excellent performance for the diagnosis of diaphragm dysfunction and severe diaphragm dysfunction with areas under the curve of 0.90 (95% CI, 0.83 to 0.97) and 0.88 (95% CI, 0.82 to 0.95), respectively. This performance was not significantly affected by the body mass index or the presence of a neck catheter.Conclusions:The SONOTEPS method is a simple and accurate tool for bedside assessment of diaphragm function with ultrasound-guided transcutaneous phrenic nerve stimulation in sedated patients with no or minimal spontaneous respiratory activity.

01 Feb 2025·Movement Disorders Clinical Practice

KCNMA1‐Related Episodes of Behavioral Arrest and Loss of Postural Reflexes: A Critical Reappraisal

Article

Author: de Gusmao, Claudio M. ; Roubertie, Agathe ; Leu‐Semenescu, Smaranda ; Villeneuve, Nathalie ; Lecardonnel, Bérénice ; François‐Heude, Marie‐Céline ; Silveira‐Moriyama, Laura ; Meyer, Pierre ; Roze, Emmanuel

AbstractBackgroundKCNMA1‐linked channelopathy is characterized by neurodevelopmental disorder, epileptic seizures and non‐epileptic paroxysmal episodes.ObjectivesTo describe the phenotype of paroxysmal non‐epileptic episodes related to KCNMA1 pathogenic variants.MethodsVideos of paroxysmal episodes were reviewed according to a standardized protocol by a group of movement disorders experts.ResultsFourteen videos were reviewed (6 previously published patients and a new patient). The typical pattern of an episode was (i) facial changes including dyskinetic movements of tongue and jaws (ii) behavioral arrest (iii) loss of postural reflexes that could be associated with focal body stiffness, eventually leading to fall (iv) rapid recovery without post‐ictal drowsiness. Attacks were brief, with a high daily frequency, occasionally triggered by emotion, and dramatically improved by psychostimulant therapy in three patients.ConclusionsKCNMA1‐related attacks are clearly distinguishable from paroxysmal dyskinesia, cataplexy or episodic ataxia indicating a unique phenomenological entity whose recognition will enhance accurate diagnosis and treatment.

100 Deals associated with The Institute For Neurosciences of Montpellier

100 Translational Medicine associated with The Institute For Neurosciences of Montpellier

Corporation Tree

Boost your research with our corporation tree data.

view full example data

Pipeline

Pipeline Snapshot as of 13 May 2025

No data posted

Deal

Boost your decision using our deal data.

view full example data

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Profit

Explore the financial positions of over 360K organizations with Synapse.

view full example data

Grant & Funding(NIH)

Access more than 2 million grant and funding information to elevate your research journey.

view full example data

Investment

Gain insights on the latest company investments from start-ups to established corporations.

view full example data

Financing

Unearth financing trends to validate and advance investment opportunities.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis