Phytopharm Kleka SA

Ellagic acid (EA) is an important compound of dietary food as an antioxidant and it was reported to have beneficial effects on human health.Optimization of sample preparation procedures and exptl. conditions for the fast and simple determination of the free and the total EA content in cloudberry's (Rubus chamaemorus L.) leaves by HPLC were developed.For the 1st time the mixture of vitamin C and δ-tocopherol was used to protect EA from the influence of oxygen and light and this combination of the two agents provided the best effect in comparison to other known antioxidants.The influence of time and temperature on EA yield in the hydrolyzed sample was also analyzed.The HPLC conditions applied resulted in a satisfactory separation of EA standard solution as well as the studied sample.One determined high concentration of the free and the total EA amount in dried leaves of R. chamaemorus, 0.137 and 6.996 g on 100 g of dry mass.