Beijing Yiling Pharmaceutical Co., Ltd.

Epiberberine, a natural bioactive protoberberine alkaloid, was totally synthesized in short, convenient and low-cost, four-step reactions including cyclization, condensation, reduction, and ring-closing, with an overall yield of 26.1%.

The best extraction process conditions of Qingyan Ziyin Capsules were optimized.The extracting frequency, extraction time and adding water amount which greatly affecting the yield rate were selected as the investigation factors and the orthogonal test was conducted with the berberine hydrochloride content as the investigation index.The optimal extraction process was to extract for 3 times, the first time was to extract for 2 h, adding 9 times amount of water and soaking for 1 h; at the second time and the third time, adding 6 times amount of water, extracting for 1.5 h and 1 h resp.The optimized extraction process cansave time and energy, it can be applied in the industrial production

Three new chromone glycosides, named 2-methyl-5-hydroxyl-4-chromone-7-O-β-D-glucuronide (1), 2-ethyl-5-hydroxyl-4-chromone-7-O-β-D-glucuronide (2) and 1-acetyl-3-C-β-D-glucopyranosyl-5-methylphloroglucinyl-6-O-β-D-glucopyranoside (6), together with eight known compounds were isolated from the water extract of Dryopteris crassirhizoma Nakai,and the water extract preparation process is consistent with Lianhua Qingwen capsule.All the chem. structures of new compounds were characterized by 1D and 2D NMR spectra as well as high resolution mass and IR spectra.The chem. structures of known compounds were characterized by NMR spectra along with comparison of their spectroscopic data with those reported in the literature.The binding ability of these compounds to 3CL hydrolase of SARS-COV-2 was evaluated by mol. docking.The results showed that the chromone glycosides with glucuronic acid fragment had good docking effect with 3CL hydrolase and this indicated the chromone glucuronides could be further studied as potential antiviral compounds