Request Demo

Last update 08 May 2025

OXSM

Last update 08 May 2025

Basic Info

Synonyms

3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial, 3-oxoacyl-ACP synthase, mitochondrial, Beta-ketoacyl-ACP synthase

+ [7]

Introduction

May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.

Drugs associated with OXSM

Platencin

Target

OXSM

Mechanism

OXSM inhibitors

Active Org.

Merck Research Laboratories Massachusetts LLC

Originator Org.

Merck Research Laboratories Massachusetts LLC

Active Indication

Bacterial Infections

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

JSF-3285

Target

OXSM

Mechanism

OXSM inhibitors

Active Org.

Rutgers State University of New Jersey

Originator Org.

Rutgers State University of New Jersey

Active Indication

Tuberculosis

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

Platensimycin

Target

OXSM

Mechanism

OXSM inhibitors

Active Org.

Merck & Co., Inc.

Originator Org.

Merck & Co., Inc.

Active Indication

Antibiotic resistant infection

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with OXSM

100 Translational Medicine associated with OXSM

0 Patents (Medical) associated with OXSM

383

Literatures (Medical) associated with OXSM

01 Jul 2025·Microbial Pathogenesis

Antilisterial activity of Thymus vulgaris essential oil: In vitro, in situ, and in silico investigations

Article

Author: Laaraj, Salah ; Shahat, Abdelaaty A ; Giarratana, Filippo ; Moujane, Soumia ; Farihi, Ayoub ; Elfazazi, Kaoutar ; Ed-Dra, Abdelaziz ; Giuffrida, Alessandro ; Noman, Omar M ; Nalbone, Luca

Listeria monocytogenes is a major foodborne pathogen that significantly threatens public health and food safety. While Thymus vulgaris essential oil (TV-EO) is widely recognized for its potent antibacterial activity, its specific effects against L. monocytogenes remain unexplored. This study aimed to assess the antilisterial activity of TV-EO using in vitro, in situ, and in silico approaches. The in vitro assessment included disc diffusion method, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), biofilm inhibition assay, and predictive modeling to assess L. monocytogenes reduction in the presence of TV-EO at 10 °C and 20 °C. In situ approach evaluated the inhibitory effect of TV-EO on L. monocytogenes in minced poultry meat stored at 4 °C. Finally, in silico approach, based on molecular docking, was employed to evaluate the binding affinity of major TV-EO components for β-ketoacyl-ACP synthase II and chorismate synthase, key proteins involved in fatty acid biosynthesis and biofilm formation, respectively. Our finding revealed that TV-EO exhibited strong in vitro antilisterial activity, with inhibitory zones ranging from 51.00 ± 1.00 mm to 55.67 ± 1.15 mm, a MIC value of 0.125 %, and a MBC value of 0.25 %, indicating its bactericidal effect. TV-EO at 0.125 % demonstrated a high capacity to inhibit and eradicate the biofilm, with 100 ± 0.00 % and 91.33 ± 1.23 %, respectively. Predictive modeling, based on the combination of TV-EO and ζ values, revealed that L. monocytogenes inactivation was more pronounced at low temperature. Furthermore, the in-situ approach showed a significant reduction of L. monocytogenes amount, with decreases of 1.068 ± 0.132 log cfu/g, 0.671 ± 0.091 log cfu/g, and 0.317 ± 0.029 log cfu/g at TV-EO concentrations of 1 %, 0.5 %, and 0.25 %, respectively (p < 0.05). In silico analysis indicated that TV-EO components, particularly carvacrol, exhibited high affinity for β-ketoacyl-ACP synthase II and chorismate cynthase, suggesting strong antilisterial and ani-biofilm activity. These findings highlight the antilisterial efficacy of TV-EO, demonstrating its potential as a natural alternative to conventional preservatives for enhancing food preservation and safety.

01 Mar 2025·Physiology and Molecular Biology of Plants

Multi-omics analysis of non-pungent (Capsicum annuum) and fiery hot ghost chili (C. chinense) provides insights into proteins involved in fruit development and metabolites biosynthesis

Article

Author: Islam, Khushbu ; Ramchiary, Nirala ; Vishesh ; Ahmad, Ilyas ; Momo, John ; Biswas, Souparna ; Rawoof, Abdul

Global omics offer extensive insights into the diversity of essential biomolecules across various plant developmental stages. Despite advancements in high-throughput technologies, the integrated analysis of global omics such as proteomics, transcriptomics, and metabolomics, is yet to be fully explored in fruits of Capsicum species. In this study, we used an integrated omics approach to identify proteins involved in fruit development, and metabolite biosynthesis in the placenta and pericarp tissues of two contrasting genotypes belonging to ghost chili (Capsicum chinense) and C. annuum. The mass spectrometry analysis identified a total of 4,473 and 2,012 proteins from the pericarp and placenta tissues of Capsicum fruits. We observed expression of developmental stage-specific proteins, such as kinases, transferases, ion transporters, F-box proteins, and transcription factors that were enriched in the biosynthesis of primary and secondary metabolites. The abundance of these proteins corresponded with RNAseq data. Key proteins related to capsaicinoids biosynthesis, such as Acyltransferase 3, 3-oxoacyl-[acyl-carrier protein], 4-coumaroyl co-A ligase, and 3-ketoacyl-coA synthase 3, were identified in placenta of highly pungent ghost chili, along with J-domain proteins and transcription factors such as MYB101, MYB 14-like, bHLH112, NAC, and Cyt p450 CYP82D47, suggesting their role in capsaicinoids and secondary metabolites biosynthesis. Further, we observed a correlation of the expression of genes and proteins with the abundance of primary and secondary metabolites, such as carbohydrates, alcohols, fatty acids, phenolics, glycerides, polyamines, and amino acids. Our findings provide a novel multiomics resources for future functional studies, with potential applications in breeding programs.Graphical AbstractSupplementary InformationThe online version contains supplementary material available at 10.1007/s12298-025-01581-7.

01 Mar 2025·Journal of Microbiological Methods

Rapid screening of primary and rationally synthesized anti-mycobacterial compounds in macrophage using double recombinant M. bovis BCG strain

Article

Author: Gunjan ; Ansari, Mohd Mustkim ; Singh, Bhupendra N ; Kumari, Garima ; Verma, Ruchi ; Dhuriya, Rajendra Kumar

Antimycobacterial screening is done primarily at three levels; in vitro, ex vivo and in vivo. In earlier studies, we had generated a double recombinant Mycobacterium bovis BCG strain carrying firefly and Renilla luciferase genes as two reporters under the control of a constitutive and an inducible mycobacterial promoter. The presence of dual reporters allows simultaneous expression and analysis of two reporter enzymes within a single system. The expression profile of the firefly luciferase gene, rendered by a constitutive mycobacterial promoter, corroborates with the decline in bacterial growth in response to a wide range of antimycobacterial drugs, while the enhanced expression of Renilla luciferase mirrors the selective induction of the reporter gene expression as a result of FAS-II pathway-specific inhibition. Thus, the double recombinant strain allows the screening of both primary and rationally synthesized FAS-II pathway inhibitors in a single assay. While this was successfully used for in vitro screening, ex vivo adaptation of this screen-system posed several challenges. The constitutive hsp60pr showed appreciable expression inside macrophages, but the expression of the inducible kas operon promoter was found to be meager. This became a limiting factor as more number of bacilli needed per screening sample and with continued treatment the decline in CFU level worsens the detection limit of the luciferase assay. To develop a screen-system that compensate the lower level expression of a given mycobacterial promoter inside macrophages we introduced Nano luciferase reporter in recombinant mycobacteria. Nano luciferase emits several-fold brighter luminescence than firefly and Renilla luciferases and duly compensates the lower level expression of the kas operon promoter inside macrophages. The newly engineered double recombinant strain stays stable inside macrophages and serves as a model screen-system for general and pathway specific anti-mycobacterial ex vivo screening. The turnaround time is significantly reduced and the outcomes are similar and more consistent with those attained using conventional CFU based procedures.

Analysis

Perform a panoramic analysis of this field.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

OXSM

Basic Info

Related

Analysis