LTB4R

NEW YORK and LONDON, July 28, 2022 (GLOBE NEWSWIRE) -- Akari Therapeutics, Plc (Nasdaq: AKTX), a late-stage biotechnology company focused on developing advanced therapies for autoimmune and inflammatory diseases, today announced positive results from recent pre-clinical studies on the tolerability and extended dose interval of long-acting PAS-nomacopan in development for geographic atrophy (GA) in dry age-related macular degeneration (dAMD). The results support the potential for PASylated nomacopan to advance toward the regulatory application(s) that would be required to begin clinical trials. “These pre-clinical results contribute to the strong foundation we are building for a potential PAS-nomacopan clinical trial program in geographic atrophy,” said Rachelle Jacques, President and CEO of Akari. “The work we have completed to show bispecific inhibition of complement C5 and LTB4 and to optimize PAS-nomacopan for dosing interval and dose volume, while achieving manufacturing scalability, positions us well to advance this program toward the clinic. We expect to have clarity on IND/IMPD timing by year end.” GA manifests as a chronic progressive degeneration of the macula, which occurs during late-stage dAMD and can lead to irreversible vision loss. Approximately five million people worldwide are affected,1 with nearly one million in the U.S.2 There are no approved treatment options. Akari’s pre-clinical research program investigating long-acting PAS-nomacopan in GA seeks to address three areas of significant unmet patient need including: providing longer intervals between intravitreal injections into the back of the eye, a dose volume that has little impact on intraocular pressure (IOP), and, through LTB4 inhibition, reduced risk of sight-threatening choroidal neovascularization (CNV), also known as wet age-related macular degeneration (wAMD), which can be a complication of certain late-stage complement-only inhibitors.3,4 An Akari pre-clinical study presented at ARVO 2022 used an industry standard model of laser induced CNV. Intravitreal (IVT) early generation PAS-nomacopan injected once during a 16-day treatment period was compared to an FDA-approved vascular endothelial growth factor (VEGF) inhibitor for impact on neovascularization. VEGF inhibitors are often used to treat CNV/wAMD. The IVT single dose of early generation PAS-nomacopan significantly reduced CNV (p=0.022) as compared to saline and was as effective as multiple IVT injections of the VEGF inhibitor (p=0.019.) The CNV data also included an interesting result. Multiple IVT doses of early generation PAS-nomacopan were less effective than the single IVT dose of PAS-nomacopan. One explanation for the result might be presence of non-endotoxin pyrogens (NEPs) in the early generation PAS-nomacopan preparation, which may cause activation of monocytes through Toll-like receptor (TLR) signaling. Analysis of the early generation PAS-nomacopan used in the CNV study showed that the preparation did contain NEPs, which prompted Akari to develop an adapted purification process that produced early generation PAS-nomacopan without NEPs that no longer activates monocytic cells and TLR2/TLR4. The NEP-free early generation PAS-nomacopan was used in a tolerability and pharmacokinetic (PK) study. A single intravitreal dose of highly purified PAS-nomacopan was shown to be free of endotoxins and non-endotoxin pyrogens and suitable for use in the eye in either low or high (3x) concentration New PK measurements from the recent pre-clinical studies support Akari’s ongoing work to engineer new generation PAS-nomacopan that has the potential to offer patients extended intravitreal dose intervals of more than three months. PK measurements indicate the predicted half-life based of early generation PAS-nomacopan matches the actual half-life within the eye of intravitreally injected NEP-free PAS-nomacopan in a standard ophthalmic pre-clinical model The new PK measurements show half-life of early generation PAS-nomacopan without NEPs in vitreous increased to seven days compared with a half-life of five days for the same PAS-nomacopan with NEPs (in data presented at ARVO 2022) The ability to accurately predict half-life supports Akari’s work to develop a new form of PAS-nomacopan that may permit longer dosing intervals of more than three months between IVT injections. Extending half-life has the potential to achieve a dose volume that has little impact on intraocular pressure (IOP). A study evaluating the patient burden of repeated IVT (median frequency of once every 4.5 weeks) found that anxiety, discomfort and disruption to normal activities and inconvenience were common experiences. The most common source of anxiety is the fear of the injection into the eye and associated discomfort. Patients related discomfort to what they felt after anesthesia wears off. Patients experiencing disruption reported being debilitated after each IVT, requiring an average of eight hours to recover.5 Occasional dystrophic retina (retinal degeneration) was observed in some test subjects in the recent pre-clinical studies and was unrelated to PAS-nomacopan as the incidence was equivalent in untreated eyes and controls as in treated test subjects. Some signs of ocular inflammation and anterior cell infiltration were observed that occurred 14 days or more after IVT administration suggesting they were not due to drug toxicity or an innate immune response, which typically peaks two to three days after IVT injection. In summary, LTB4 signaling has shown to be involved in VEGF expression,6,7 which is a key driver of the damaging angiogenesis (blood vessel development) and retinal inflammation in CNV/wAMD. The nomacopan bispecific inhibition of both complement C5 and LTB4 may be a novel approach to treating GA/dAMD, while potentially helping to reduce the sight-threatening risk of CNV/wAMD. Akari’s pre-clinical studies for PAS-nomacopan in GA are conducted in collaboration with ophthalmology specialty contract research organization IRIS Pharma.