ERK x RHOA x TGF-β1 x AXL x ROCK

Last update 08 May 2025

Basic Info

Related Targets

ERKRHOATGF-β1[+2]

Drugs associated with ERK x RHOA x TGF-β1 x AXL x ROCK

Xihuang pill(Beijing University of Chinese Medicine)

Target

AXL x ERK x RHOA x ROCK x TGF-β1

Mechanism

AXL modulators [+4]

Active Org.

Beijing University of Traditional Chinese Medicine

Originator Org.

Beijing University of Traditional Chinese Medicine

Active Indication

Triple Negative Breast Cancer

Inactive Indication-

Drug Highest PhasePreclinical

First Approval Ctry. / Loc.-

First Approval Date20 Jan 1800

100 Clinical Results associated with ERK x RHOA x TGF-β1 x AXL x ROCK

100 Translational Medicine associated with ERK x RHOA x TGF-β1 x AXL x ROCK

0 Patents (Medical) associated with ERK x RHOA x TGF-β1 x AXL x ROCK

Literatures (Medical) associated with ERK x RHOA x TGF-β1 x AXL x ROCK

01 Mar 2025·Journal of Ethnopharmacology

Xihuang pill suppresses triple-negative breast cancer via regulating AXL/TGF-β1/RhoA/ROCK and ERK pathway

Article

Author: Bi, Yu ; Naseem, Anam ; Yang, Haolin ; Wang, Min ; Huang, Jinchang ; Liu, Yan

ETHNOPHARMACOLOGICAL RELEVANCEXihuang pill (XHP), a traditional prescription for treating "Ru yan (breast cancer)" for 270 years, has the efficacies of clearing heat and improving blood circulation. Previous research has demonstrated that XHP yields significant therapeutic effects in the management of triple-negative breast cancer (TNBC).AIM OF THE STUDYTo explore and validate the usage of a traditional Chinese medicine formula XHP in combating TNBC by epithelial-mesenchymal transition (EMT) and apoptosis mechanisms.MATERIALS AND METHODSThe cytotoxic effects of XHP were evaluated using the CCK-8 assay after incubating with 4T1 and MDA-MB-231 TNBC cell lines. WB assay was conducted to detect the expression of proteins associated with EMT and apoptosis signaling pathways, elucidating the mechanism by which XHP inhibits TNBC. DAPI staining, and mitochondrial membrane potential (MMP) assays (JC-1) were utilized to further confirm the effect of apoptosis in TNBC. Moreover, morphological examination, wound healing assays, and transwell experiments were used to investigate the EMT properties of XHP. Besides, this study constructed 4T1-xenograft model to assess the anti-tumor efficacy of XHP, while the underlying mechanism was revealed by H&E staining.RESULTSXHP suppressed tumor growth and metastasis in Balb/c transplanted tumor model by inducing EMT and apoptosis. XHP modulated the expression of the AXL/TGF-β1/RhoA/ROCK pathway, reducing tumor cell EMT, and influencing the ERK signaling pathway. Moreover, XHP inhibited the expression level of Bcl2, Bax and caspase 9 to promote apoptosis in tumor cells. These phenomena eventually manifested as inhibiting the proliferation and migration of TNBC cells.CONCLUSIONSXHP inhibits TNBC and regulates EMT and apoptosis phenotypes by regulating the AXL/TGF-β1/RhoA/ROCK and ERK pathways. Therefore, it could be further utilized in the discovery of anti-cancer drugs.

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