Delving into the Latest Updates on Avicenna Research Institute with Synapse

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Introduction

The Avicenna Research Institute (ARI) is an organization that was founded in 1998 with the aim of advancing medical technology through clinical and laboratory research projects. It is affiliated with the ACECR and consists of three research centers: the Reproductive Biotechnology Research Center (RBRC), Monoclonal Antibody Research Center (MARC), and Nanobiotechnology Research Center (NBRC). Additionally, ARI houses the Avicenna Biotechnology Incubator and Avicenna Infertility Clinic (AIC).

The catalytic performance of Candida antarctica lipase B (CALB) immobilized on silica-coated magnetic nanoparticles was evaluated for biodiesel production via methanolysis of rapeseed oil. Two different covalent immobilization approaches were compared to assess the effect of immobilization protocols on lipase efficiency. The first approach involved immobilization of CALB on amine-functionalized magnetic nanoparticles (MNPs), which targeted the Lys-rich regions of the enzyme. The second used epoxy-functionalized MNPs, enabling broader nucleophilic groups on the enzyme surface to participate in the coupling reaction. Immobilization of 20 mg of CALB on 1 g of each support resulted in 82 % and 86 % protein loading on the amine- and epoxy-functionalized MNPs, respectively, after 24 h of incubation. Response surface methodology (RSM) was applied to optimize biodiesel production by analyzing the effects of parameters such as reaction temperature, time, t-butanol concentration, biocatalyst loading, and molecular sieve quantity on the yield of fatty acid methyl esters (FAME). Out of 45 designed experiments, the maximum FAME yields were 92 % and 84 % for the epoxy- and amine-functionalized MNPs, respectively.

01 Jan 2025·Analytical Biochemistry

Production and characterization of a panel of anti-mouse placenta-specific protein 1 (plac1) monoclonal antibodies

Article

Author: Shokri, Fazel ; Zarnani, Amir-Hassan ; Amiri, Mohammad Mehdi ; Mortezagholi, Sahar ; Ghorbani, Ahmad ; Maghsood, Faezeh ; Shabani, Mahdi ; Shojaeian, Sorour

Placenta-Specific Protein 1 (PLAC1) is essential for normal placental and embryonic development. It is widely expressed in various types of cancer cells. We produced a panel of anti-mouse plac1 monoclonal antibodies (mAbs) with different applications. Two recombinant proteins were produced containing either the extracellular domain (ED) plus tetanus toxin P2, P30, pan-DR epitope (PADRE), and KDEL3 (main plac1) or ED plus KDEL3 (control plac1). Recombinant proteins were used for immunization and screening. Positive clones were selected by ELISA and flow cytometry. Purified mAbs were tested by ELISA, WB, flow cytometry, immunohistochemistry (IHC), and immunofluorescent (IF). A combination of bioinformatics tools was used to predict the target epitope(s) of the mAbs. Eight anti-mouse plac1 mAbs (all IgG1/κ1) were generated, all reacting with high affinity in ELISA. Seven clones recognized plac1 in both reduced and non-reduced Western blots, while one only recognized the non-reduced form. Cross-inhibition ELISA revealed that all mAbs recognized overlapping epitopes with a shared motif except for 5C9. Four clones reacted with the native antigen in flow cytometry, but none were functional in IF or IHC staining. The produced multifunctional mAbs can be used to investigate different aspects of PLAC1 biology in reproduction and cancer.

31 Dec 2024·Future Science OA

Nanofabrication of chitosan-based dressing to treat the infected wounds: in vitro and in vivo evaluations

Article

Author: Fatideh, Fereshteh Mohammadi ; Najafzadeh, Somayeh ; Nejadmoghaddam, Mohammad-Reza ; Minai-Tehrani, Arash ; Mohammadi, Zohreh ; Ghahremanzadeh, Ramin ; Yousefi, Maryam ; Samadi, Fatemeh Yazdi

Aim: Here, an innovative kind of antibacterial nanocomposite film is developed by incorporating graphene oxide and zinc oxide into chitosan matrix. Materials & methods: Our dressing was fabricated using the solution casting method. Fourier transform infrared spectra and TGA-DTG clearly confirmed the structure of film dressing. Results & conclusion: Our results showed the tensile strength and elongation at the break of the films were 20.1 ± 0.7 MPa and 36 ± 10%, respectively. Our fabricated film could absorb at least three-times the fluid of its dry weight while being biocompatible, antibacterial, non-irritant and non-allergic. In addition, it accelerated the healing process of infected wounds by regulating epithelium thickness and the number of inflammatory cells, thus it may be useful for direct application to damaged infected wounds.

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100 Translational Medicine associated with Avicenna Research Institute

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Pipeline

Pipeline Snapshot as of 03 Feb 2025

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

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