The method for the determination of the total flavonoids in Naoxinqing dripping pills was established.UV spectrophotometry with the detection wavelength of 250 nm was used for the determination with rutin as the referenceThe linear range of the total flavonoids was 6.48-32.48 μg/mL (r = 0.9993).The average recovery rate of the total flavonoids was 99.78% with RSD of 1.86%.This method was accurate, reproducible, simple, and suitable for the quality control of Naoxinqing dripping pills.