A method for determination of puerarin in Xingmaitong drop pills was established. HPLC was used, the separation was performed on Luna C18 (5 μg, 250 mm×4.6 mm) column, a mixture of methanol-water (25:75) was served as the mobile phase, and the detecting wavelength was at 2250 nm. The linearity range was from 4.16-83.2 mg/L, and the mean recovery was 100.84%, with RSD 1.06%. The method is simple, accurate and good for the determination of puerarin in Xingmaitong drop pills, which can be used for quality control of the preparation