SPH No. 1 Biochemical & Pharmaceutical Co., Ltd.

Myocardial infarction (MI) leads to pathological cardiac remodeling and heart failure. Sodium tanshinone IIA sulfonate (STS) shows to possess therapeutic potential. The present study aimed to explore the potential role of STS in ventricular remodeling post-MI.

Mice were randomly divided into sham, MI + normal saline (NS) and MI + STS (20.8 mg/kg/day intraperitoneally) groups. MI was established following left anterior descending artery ligation. Cardiac function was evaluated using echocardiography. Scar size and myocardial fibrosis-associated markers were detected using Masson’s trichrome staining and western blot analysis (WB). Necrosis and inflammation were assessed using H&E staining, lactate dehydrogenase (LDH) detection, ELISA, immunohistochemical staining, and WB. Furthermore, angiogenesis markers and associated proteins were detected using immunohistochemical staining and WB.

Mice treated with STS exhibited significant improvements in cardiac function, smaller scar size, and low expression levels of α-smooth muscle actin and collagen I and III at 28 days following surgery, compared with the NS-treated group. Moreover, treatment with STS reduced eosinophil necrosis, the infiltration of inflammatory cells, plasma levels of LDH, high mobility group protein B1, interleukin-1β and tumor necrosis factor- α, and protein expression of these cytokines at 3 days. Macrophage infiltration was also decreased in the STS group in the early phase. Additionally, CD31+ vascular density, protein levels of hypoxia-inducible factor- 1α, and vascular endothelial growth factor were elevated in the STS-treated mice at 28 days.

STS improved pathological remodeling post-MI, and the associated therapeutic effects may be a result of a decrease in myocardial necrosis, modulation of inflammation, and an increase in angiogenesis.

Objective: To prepare high-purity trypsin and investigate its quality.Methods: The manufacturing process for the production of trypsin included dissolving trypsinogen(crude extract), inactivating virus by incubation at low p H value, desalinating by ultra-filtration, trypsinogen activating, purification by affinity chromatog., eluent desalinating by ultrafiltration, virus removal by nano-film filtration and vacuum freeze-drying.Results: The prepared trypsin could reach to the standard of pharmacopeia.Conclusion: The production cycle can be shortened, the potency of trypsin and the stability of the process can be improved, the content of chymotrypsin can be reduced and the quality and safety of trypsin can be increased.

Vitamin K₁ with different ratio of cis/trans isomers was synthesized from natural phytol via bromination with phosphorus tribromide in the presence of different content of water to get different ratio of (E/Z)-isomeric phytyl bromide, followed by condensation with cyclopentadiene-menadione adduct and retro-Diels-Alder reaction.