Guangzhou Baiyunshan Guang Hua Pharmacy Co., Ltd.

Two new sucrose cinnamates(1 and 2) along with nine known compounds(3-11) were isolated from ethanol extract of Polygonum lapathifolium var. salicifolium by silica gel column chromatog., ODS column chromatog. and semi-preparative HPLC.Their structures were elucidated by extensive spectroscopic methods including 1 D-and 2 D-NMR experiments, as well as HR-ESI-MS anal.Eleven compounds(7 sucrose cinnamates, 3 phenylpropanoids and 1 lactone) were obtained and their structures were identified as(1, 3-O-di-p-coumaroyl)-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside(1), (1, 3-O-di-p-coumaroyl)-β-D-fructofuranosyl-(2→1)-(6-O-acetyl)-α-D-glucopyranoside(2), (3-O-feruloyl)-β-D-fructofuranosyl-(2→1)-(6-O-p-coumaroyl)-α-D-glucopyranoside(3), hydropiperoside(4), vanicoside C(5), (1, 3-O-di-p-coumaroyl)-β-D-fructofuranosyl-(2→1)-(6-O-feruloyl)-α-D-glucopyranoside(6), vanicoside B(7), trans-p-hydroxycinnamic acid Me ester(8), trans-p-hydroxycinnamic acid Et ester(9), Me ferulate(10) and dimethoxydimethylphthalide(11), resp.Compounds 1 and 2 were two new sucrose cinnamates, and compounds 1-11 were isolated from this plant for the first time.The antioxidant activities of the isolated compounds 1-9 were investigated by an oxygen radical absorbance capacity(ORAC) assay, and all nine compounds were found to show strong antioxidant activities.Among them, compound 6(10 μmol·L∼(-1)) was the supreme one in antioxidant activities, with its ORAC value equivalent to(1.60±0.05) times of 50 μmol·L∼(-1) Trolox.

Objective To explore the mol. mechanism of antipyretic effect of Xiaochaihu Granules(XCHG).Methods The chem. components of XCHG were separated and identified by fingerprint and UHPLC-MS technol.The targets of components were obtained and classified using PubChem, Swiss and chEMBL databases.The targets of fever were obtained from GeneCard and DisGeNET database.The drug targets and fever targets were intersected, the antipyretic targets database of XCHG was structured and the functional enrichment was analyzed.The model of rats induced by dried yeast was established, and the temperature of rats was monitored within 24 h and the content of cytokines in serum was detected.Results 66 chem. components and 68 potential antipyretic targets of XCHG was screened out.Enzymes, including kinase, protease, oxidoreductase and phosphatase, were the most widely classified category.Compared with aspirin, XCHG could significantly reduce the body temperature of rats with fever at 4∼24 h after modeling, and the effect of medium dose and high dose were similar to that of aspirin.XCHG could significantly reduce the content of TNF-α, IL-6, IFN-γ, IL-1 β and PTGS2, also could decreased the lever of NF-κB and AP-1.Conclusion XCHG can significantly lower the body temperature of rats with fever induced by dried yeast in the rising, peak and falling periods.The antipyretic mechanism of XCHG is related to many links of fever, which may be related to reducing the level of inflammation, inhibiting endogenous pyrogen and PTGS2.

OBJECTIVE To establish the dissolution test method and investigate the quality consistence of potassium iodide tablets by comparative anal. of the dissolution curve with the original drugs.METHODS The paddle dissolution method was used for the dissolution test with water as the dissolution medium, and the rotation rate was set at 50 r · min^-1.The UV-visible spectroscopy was used to determine the concentration of potassium iodide and the detection wavelength was set at 227 nm.RESULTS There was a good linear relationship in the range of 1-7μg · mL^-1 for potassium iodide (r = 0.9999), and the average recovery was 99.78% (RSD = 0.87%).CONCLUSION The established anal. method is specific, simple, accurate and with high sensitivity.The determined dissolution of potassium iodide tablets meet the requirements and the dissolution curve is similar to that of the original drugs.The established method can be applied for the quality consistence evaluation of potassium iodide tablets.