Acetylated distarch adipates was prepared by cassava starch with adipic acetic mixed anhydride. It showed that the optimal parameters were initial starch slurry concentration 30%, pH 8, reaction time 1.5 h and mixed anhydride concentration 4.5%, resp. The degree of hydroxypropyl substitution was 0.1264, mixed anhydride molar substitution degree was 0.031. The properties of retrogradation, freeze-thaw stability and the degree of clarity were studied, retrogradation and freeze-thaw stability of acetylated distarch adipates had been greatly improved, and solubility and transparency were slightly lower than cassava starch.