Objective:To prepare Hydroxycamptothecin liposome and observe its anti-tumor effect on H_(22) hepatoma and S_(180) sarcoma animal models in mice. Methods: Hydroxycamptothecin liposome was prepared usingfilm-dispersion method, and its characteristic such as particle size, was studied. The H_(22) hepatoma and S_(180) sarcoma models were established in KM mice. They were injected i.v. every other day for three times. On the fifth day after treatment, the animals were killed, and blood samples were collected to count white blood cells, and the s.c. sarcoma were separated and weighted. Tumor inhibition rate were calculated Results: The size of the Hydroxycamptothecin liposome was 357 nm. The encapsulation efficiency was more than 93%, and the ratio of active form was more than 98%. Hydroxycamptothecin liposome could inhibit the growth of both H_(22 )hepatoma and S_(180 )sarcoma tumor, At the same dose, the differences were statistically significant between Hydroxycamptothecin liposome groups and Hydroxycamptothecin injection(P<0.001). White blood cells were reduced to varying degrees in all treatment groups and the differences were statistically significant between control group and treatment groups. Conclusion: Hydroxycamptothecin liposome con inhibit the growth of tumor.