Gonadorelin Acetate

The main objectives of the present study were to determine the effects of presynchronizing with a 1.0 g intravaginal progesterone device (IVPD) and prostaglandin F2α and to assess the effects of re-utilization of IVPD in a 2x2 factorial design, on the ovulatory response to first GnRH, ovarian status at different protocol stages, estrus expression and fertility in beef heifers submitted to a 5d-CO-Synch + Progesterone (P4) protocol. Beef heifers (n = 564) were assigned to 1 of 2 treatments at D-15: Pres5 (n = 283), where heifers received a (IVPD) for 5 days and administration of prostaglandin F2α (25 mg of dinoprost) at D-10; and Control (n = 281), where heifers received no treatment. At D-8, all heifers received 100 μg of GnRH (gonadorelin acetate) and were assigned to 1 of 2 IVPD-use treatments: new-IVPD (n = 279), where animals received a new IVPD for 5 days, and once-used IVPD (n = 285), where heifers received a once-used IVPD for 5 days (used previously for 5 days). On D-3, IVPD was removed and 50 mg of prostaglandin F2α was administered. All heifers were timed artificially inseminated (AI; D0) 62 h after IVPD removal concomitant with an administration of 100 μg of GnRH. Estrus detection patches were placed on heifers at D-3 and evaluated at the time of AI. In a subset of heifers (n = 278), transrectal ultrasonography of the ovaries was carried out at D-8 and D-3 to assess presence and diameter of CL and largest follicle diameter (LFD). A blood sample was collected on D-3 to determine serum P4 concentration. Heifers with a once-used IVPD had a greater (P = 0.01) pregnancy per AI (P/AI) than heifers treated with a new-IVPD [62.8 % (179/285) vs 51.2 % (143/279)]. There was a lower percentage of animals (P = 0.002) having a corpus luteum (CL) on D-8 in Pres5 group compared to Control [16.4 % (22/134) vs 69.4 % (100/144)]. Similarly, a greater percentage of Pres5 heifers had a dominant follicle on D-8 (P < 0.0001) than Control heifers [97.7 % (131/134) vs 75.7 % (109/144)]. At D-3, Pres5 heifers had a greater ovulatory response to D-8 GnRH (P < 0.0001) compared to Control animals [82.8 % (111/134) vs 49.3 % (71/144)]. In addition, Pres5 heifers had a greater estrus expression behavior [87.6 % (248/283) vs 72.9 % (205/281); P < 0.0001] and tended to have (P = 0.10) a greater P/AI [61.8 % (175/283) vs 52.3 % (147/281)] than Control heifers. In conclusion, the tendency for a greater fertility observed in Pres5 heifers (∼10 %) justifies the extra animal handling required for presynchronization. A once-used IVPD represents a viable strategy to enhance P/AI and reduce the cost in beef heifers submitted to timed AI.