Objective To determine the maximum allowable residue limit of Paricalcitol Injection on pharmaceutical equipment and to develop a new cleaning anal. method for testing the paricalcitol residual. Methods The chromatog. column was Kromasil 100-C18 column( 250 mm x 4. 6 mm,5 μm),the mobile phase was 15% acetonitrile-acetonitrile,with a gradient elution rate of 1. 0 m L/min,the column temperature was 25 °C,and the detection wavelength was 252 nm. Results The maximum allowable residue limit was0. 13 ng/cm2; the limits of detection and quantitation were 0. 059 ng and 0. 198 ng,resp.,the mass concentration of paricalcitol had good linear relationship with the peak area in the range of 1. 98-33. 02 ng/m L( r = 0. 999 9,n = 6). Different materials( including stainless steel,plexiglass,tempered glass,and polytetrafluoroethylene) were wiped with the same wiping method and recovered. The recovery rates were 62. 4%-98. 2%,the RSDs of the precision,repeatability,and reproducibility tests were all less than 10. 0%,the reference solution and the sample solution were stable at 5 °C for 7 d. Conclusion This method has high sensitivity,strong specificity and good accuracy,which can be used to evaluate the cleaning effect of equipment after the production of Paricalcitol Injection.